Molecular cloning and characterization of prophenoloxidase in the black tiger shrimp, Penaeus monodon

A cDNA encoding shrimp, Penaeus monodon, prophenoloxidase (proPO) was obtained by screening a hemocyte library by plaque hybridization using a proPO cDNA fragment from freshwater crayfish, Pacifastacus leniusculus, as a probe. The 3,002 bp cDNA contains an open reading frame of 2,121 bp and a 881 bp...

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Bibliographic Details
Published in:Developmental and comparative immunology 1999-04, Vol.23 (3), p.179-186
Main Authors: Sritunyalucksana, Kallaya, Cerenius, Lage, Söderhäll, Kenneth
Format: Article
Language:English
Subjects:
ACTIVATING SYSTEM
AMINO-ACID-SEQUENCE
Animals
Arthropod immunity
Base Sequence
Biologi
Biology
BLOOD-CELLS
Blotting, Northern
CALIFORNIENSIS HOLMES
Catechol Oxidase - classification
Catechol Oxidase - genetics
Cloning, Molecular
CRAYFISH PACIFASTACUS-LENIUSCULUS
DNA, Complementary
Enzyme Precursors - classification
Enzyme Precursors - genetics
Hemocyanin
INVERTEBRATE IMMUNITY
Marine
Molecular Sequence Data
NATURAL SCIENCES
NATURVETENSKAP
Penaeidae - enzymology
Penaeus monodon
PRO-PHENOL OXIDASE
prophenol oxidase
Prophenoloxidase
Sequence Analysis
Sequence Homology, Amino Acid
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Summary:A cDNA encoding shrimp, Penaeus monodon, prophenoloxidase (proPO) was obtained by screening a hemocyte library by plaque hybridization using a proPO cDNA fragment from freshwater crayfish, Pacifastacus leniusculus, as a probe. The 3,002 bp cDNA contains an open reading frame of 2,121 bp and a 881 bp 3′-untranslated region. The molecular mass of the deduced amino acid sequence (688 amino acids) is 78,700 Da with an estimated pI of 5.8. Two putative copper binding sites are present and they have a highly conserved sequence around these sites. No signal peptide was detected in the shrimp proPO, as has been previously shown to be the case for all arthropod proPOs cloned so far. The cleavage site of zymogen activation is likely to be between Arg 44 and Val 45. A tentative complement-like motif (GCGWPQHM) is also present. Shrimp proPO mRNA is synthesized in the hemocytes and not in the hepatopancreas. Comparison of amino acid sequences showed that shrimp proPO is more closely related to another crustacean proPO, namely crayfish, than to the insect proPOs.
ISSN:0145-305X
1879-0089
1879-0089
DOI:10.1016/S0145-305X(99)00005-1