Microsatellites in the sand lizard (Lacerta agilis): description, variation, inheritance, and applicability

We developed microsatellite markers for the sand lizard (Lacerta agilis) to enable investigations of the genetic variability within and among populations with a heterogeneous spatial distribution in Sweden. The populations, which could not be characterized by variation in allozymes or mitochondrial...

Full description

Saved in:
Bibliographic Details
Published in:Biochemical genetics 1997-08, Vol.35 (7-8), p.281-295
Main Authors: Gullberg, A, Tegelström, H, Olsson, M
Format: Article
Language:English
Subjects:
Alleles
Animals
Differentiation
DNA Primers
Female
Gene Library
Genetic markers
Genetic Variation
Genetics
Heredity
Isoenzymes
Lacerta agilis
Lacertilia
Lizards - genetics
Male
Maps as Topic
Microsatellite Repeats
Microsatellites
Mitochondrial DNA
Mutation rates
Paternity
Polymorphism, Genetic
Sand
Sequence Analysis, DNA
Spatial distribution
Sweden
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We developed microsatellite markers for the sand lizard (Lacerta agilis) to enable investigations of the genetic variability within and among populations with a heterogeneous spatial distribution in Sweden. The populations, which could not be characterized by variation in allozymes or mitochondrial DNA, had a substantial level of variability in microsatellite loci. However, the variability in Swedish populations was limited compared to a large, outbred Hungarian population. In the sand lizard, the number of (GT/CA)n repeats was approximately three times higher than that for (CT/GA)n. The number of repeats and the frequency of microsatellites were within the range reported for other species. Three of nine microsatellite loci showed alleles that could not be amplified, which is in agreement with recent reports describing microsatellite "null alleles" as a common occurrence. We discuss the caution which this calls for when calculating paternity probabilities and when estimating between-population allelic differentiation. A potential problem with different mutation rates for alleles within the same locus is discussed.
ISSN:0006-2928
1573-4927
DOI:10.1023/a:1021801217185