Experimental infection of white spot syndrome virus in freshwater crayfish Pacifastacus leniusculus

The signal freshwater crayfish Pacifastacus leniusculus was found to be susceptible to infection with white spot syndrome virus (WSSV). Histopathological observations of various tissues of virus-injected crayfish showed similar symptoms to those from WSSV-infected penaeid shrimp, but no appearance o...

Full description

Saved in:
Bibliographic Details
Published in:Diseases of aquatic organisms 2001-11, Vol.47 (2), p.151-157
Main Authors: JIRAVANICHPAISAL, Pikul, BANGYEEKHUN, Eakaphun, SÖDERHÄLL, Kenneth, SÖDERHÄLL, Irene
Format: Article
Language:English
Subjects:
Animal aquaculture
Animal productions
Animals
Astacoidea - virology
Biological and medical sciences
Cell Count - veterinary
Crustacea
DNA Viruses - genetics
DNA Viruses - isolation & purification
DNA Viruses - pathogenicity
DNA Viruses/genetics/isolation & purification/pathogenicity
DNA, Viral - chemistry
DNA, Viral - isolation & purification
Fresh Water
Freshwater
Fundamental and applied biological sciences. Psychology
Gills - virology
Hemocytes - cytology
Hemocytes - virology
Hemocytes/cytology/virology
In Situ Hybridization
Invertebrate aquaculture
Pacifastacus leniusculus
Polymerase Chain Reaction - veterinary
Sequence Analysis, DNA - veterinary
White spot syndrome virus
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The signal freshwater crayfish Pacifastacus leniusculus was found to be susceptible to infection with white spot syndrome virus (WSSV). Histopathological observations of various tissues of virus-injected crayfish showed similar symptoms to those from WSSV-infected penaeid shrimp, but no appearance of white spots on the cuticle or reddish body colour were observed, although these are the prominent gross signs of white spot disease in shrimp. A gene probe for detecting WSSV was developed in order to detect the virus in affected cells and tissues using in situ hybridisation. Strong signals were observed in cells of virus-injected crayfish, but not in control-injected crayfish. The number of granular haemocytes in virus-injected crayfish was significantly higher than in sham-injected and non-injected crayfish from Days 5 to 8 (p < or = 0.05) and Days 3 to 8 (p < 0.01) post-injection, respectively. The proportion of granular haemocytes in virus-injected crayfish was also significantly higher than in sham-injected controls from Days 3 to 8 (p < 0.01). These results indicate that WSSV has a significant effect on the proportion of different haemocyte types in the freshwater crayfish.
ISSN:0177-5103
1616-1580
DOI:10.3354/dao047151