Heparin coating durability on artificial heart valves studied by XPS and antithrombin binding capacity

The durability and functionality of a heparin coating on artificial heart valve leaflets were evaluated with X-ray photoelectron spectroscopy (XPS) and by the coatings’ capacity to bind antithrombin. Current methods for accelerated life-time testing are based on exposing leaflets to water solutions....

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Bibliographic Details
Published in:Colloids and surfaces, B, Biointerfaces B, Biointerfaces, 2006-04, Vol.49 (1), p.1-7
Main Authors: Kristensen, E.M.E., Larsson, R., Sánchez, J., Rensmo, H., Gelius, U., Siegbahn, H.
Format: Article
Language:English
Subjects:
Antithrombins - chemistry
Antithrombins - metabolism
Antithrombins/chemistry/metabolism
Biocompatibility
Biocompatible Materials - chemistry
Carbohydrate Conformation
Carbohydrate Sequence
Chemistry
Electrons
Heart valve
Heart Valves - chemistry
Heart Valves - metabolism
Heart Valves/chemistry/metabolism
Heparin - chemistry
Humans
Immobilized heparin
Kemi
Materials Testing
MEDICIN
MEDICINE
Molecular Sequence Data
Photoelectron spectroscopy
Sensitivity and Specificity
Spectrum Analysis
Surface characterization
Surface Properties
X-Rays
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Summary:The durability and functionality of a heparin coating on artificial heart valve leaflets were evaluated with X-ray photoelectron spectroscopy (XPS) and by the coatings’ capacity to bind antithrombin. Current methods for accelerated life-time testing are based on exposing leaflets to water solutions. In this paper a method is explored, in which heart valve leaflets were exposed to a continuous high shear rate (4 L/min) of human citrated plasma. It was found that the heparin coating was stable and wear resistant enough to still be present after 3 weeks and to have about the same antithrombin uptake as coatings not exposed to circulating plasma. It was, however, partly destroyed by the test as found using XPS. We suggest that heparin chains from the upper layer of heparin have been torn off from the carrier chain, in combination with loss of heparin conjugate and plasma deposition in patches. This study showed that XPS provides additional information to biological measurements such as antithrombin uptake. XPS is therefore a valuable technique not only to characterize biomaterials but also to evaluate the effect of a performance test.
ISSN:0927-7765
1873-4367
1873-4367
DOI:10.1016/j.colsurfb.2006.02.007