Interactions between model membranes and lignin-related compounds studied by immobilized liposome chromatography

In order to elucidate the modes of interaction between lignin precursors and membranes, we have studied the influence of temperature, lipid composition and buffer composition on the partitioning of monolignol and dilignol model substances into phospholipid bilayers. The partitioning was determined b...

Full description

Saved in:
Bibliographic Details
Published in:Biochimica et biophysica acta 2006-05, Vol.1758 (5), p.620-626
Main Authors: Boija, Elisabet, Johansson, Gunnar
Format: Article
Language:English
Subjects:
Biochemistry
Biokemi
Chemistry
Chromatography, Liquid - methods
Immobilized-liposome chromatography
Kemi
Lignin
Liposomes
membrane
Membranes, Artificial
Models, Theoretical
Monolignol
NATURAL SCIENCES
NATURVETENSKAP
Octanol/water partitioning
Phospholipid bilayers
plant
Thermodynamics
transport
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:In order to elucidate the modes of interaction between lignin precursors and membranes, we have studied the influence of temperature, lipid composition and buffer composition on the partitioning of monolignol and dilignol model substances into phospholipid bilayers. The partitioning was determined by immobilized liposome chromatography, which is an established method for studies of pharmaceutical drugs but a new approach in studies of lignin synthesis. The temperature dependence of the retention and the effect of a high ammonium sulfate concentration in the mobile phase demonstrated that the interaction involved both hydrophobic effects and polar interactions. There was also a good correlation between the partitioning and the estimated hydrophobicity, in terms of octanol/water partitioning. The partitioning behavior of the model substances suggests that passive diffusion over the cell membrane is a possible transport route for lignin precursors. This conclusion is strengthened by comparison of the present results with the partitioning of pharmaceutical drugs that are known to pass cell membranes by diffusion.
ISSN:0005-2736
0006-3002
1879-2642
1879-2642
DOI:10.1016/j.bbamem.2006.04.007