Purification, Characterization, and Biological Compartmentalization of Rat Fetal Antigen 1

This study has established the rat as an animal model for the analysis of the biological role of fetal antigen 1 (FA1), a protein previously described in humans and mice. FA1 was purified from rat amniotic fluid by immunospecific affinity chromatography. Immunochemical identity between mouse and rat...

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Bibliographic Details
Published in:Biology of reproduction 2000-07, Vol.63 (1), p.30-33
Main Authors: CARLSSON, H.-E, PERSDOTTER-HEDLUND, G, FRIES, E, ERIKSSON, U. J, HAU, J
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Amino Acids - analysis
Amniotic Fluid - immunology
Animals
Animals, Newborn
Biological and medical sciences
Cell Compartmentation
conceptus
Cross Reactions
developmental biology
Electrophoresis - methods
Female
Fetal Blood - immunology
Fundamental and applied biological sciences. Psychology
Glycoproteins - chemistry
Glycoproteins - isolation & purification
Glycoproteins - metabolism
Humans
Mice
Molecular Sequence Data
Mother. Fetoplacental unit. Mammary gland. Milk
Pregnancy
Pregnancy, Animal - immunology
Pregnancy. Parturition. Lactation
Rats
Rats, Sprague-Dawley
Sequence Homology, Amino Acid
Vertebrates: reproduction
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Summary:This study has established the rat as an animal model for the analysis of the biological role of fetal antigen 1 (FA1), a protein previously described in humans and mice. FA1 was purified from rat amniotic fluid by immunospecific affinity chromatography. Immunochemical identity between mouse and rat FA1 was established by crossed tandem immunoelectrophoresis. Molecular size was analyzed by mass spectrometry (33 kDa). The amino acid composition was determined, and the amino acid sequence was analyzed. The overall amino acid composition and sequence of the 28 first N-terminal amino acids were identical to the corresponding parts of rat preadipocyte factor 1 and rat adrenal zona glomerulosa protein. Extensive sequence similarity was found between rat and mouse FA1 (86%) and between rat and human FA1 (82%). The concentration of FA1 in fetal serum, maternal serum, urine, and amniotic fluid in rats was determined using an ELISA. The highest concentrations were found in fetal serum and amniotic fluid around Day 18 of pregnancy. This is the first report on the physicochemical characteristics and compartmentalization of rat FA1.
ISSN:0006-3363
1529-7268
1529-7268
DOI:10.1095/biolreprod63.1.30