A New Method for Incorporating Functional Heparin onto the Surface of Islets of Langerhans

A novel technique is described to conjugate macromolecular heparin complexes to cell surfaces. The method is based on the dual properties of avidin-expressing binding sites for both biotin and a macromolecular complex of heparin. A quartz crystal microbalance with dissipation monitoring (QCM-D) reve...

Full description

Saved in:
Bibliographic Details
Published in:Tissue engineering. Part C, Methods Methods, 2008-06, Vol.14 (2), p.141-147
Main Authors: Cabric, Sanja, Eich, Torsten, Sanchez, Javier, Nilsson, Bo, Korsgren, Olle, Larsson, Rolf
Format: Article
Language:English
Subjects:
Analysis
Avidin - chemistry
Binding Sites
Biomedical materials
Biotin - chemistry
Blood Coagulation
Cellular biology
Flow Cytometry - methods
Heparin
Heparin - chemistry
Humans
Immune system
Immunity, Innate
Inflammation
Islets of Langerhans - metabolism
MEDICIN
MEDICINE
Methods
Microscopy, Confocal - methods
Natural immunity
Pancreas
Physiological aspects
Surface Properties
Thrombin - chemistry
Time Factors
Tissue engineering
Tissue Engineering - methods
Transplants & implants
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A novel technique is described to conjugate macromolecular heparin complexes to cell surfaces. The method is based on the dual properties of avidin-expressing binding sites for both biotin and a macromolecular complex of heparin. A quartz crystal microbalance with dissipation monitoring (QCM-D) revealed sequential binding of biotin, avidin, and heparin complexes. Large particle flow cytometry confirmed functional integrity. Confocal microscopy of the heparinized islets showed evenly distributed fluorescence. An in vitro Chandler loop model demonstrated that the biocompatibility of the new method is comparable to the previous method used on artificial materials with regard to coagulation and antithrombin uptake. The technique presented allows human islets of Langerhans to successfully be covered with functional heparin as a means to reduce instant blood-mediated inflammatory reactions induced by the innate immune system.
ISSN:1937-3384
1937-3392
DOI:10.1089/ten.tec.2007.0312