Injectable cell-free template for bone-tissue formation

Here we present a novel injectable hydrogel which forms a template for de novo formation of bone tissue. Hydrogel formation takes place in situ in less than 1 min by the cross‐linking of multifunctional hyaluronic acid and polyvinyl alcohol derivatives. Endogenous cells are recruited in vivo by inco...

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Bibliographic Details
Published in:Journal of biomedical materials research. Part A 2009-12, Vol.91A (4), p.1111-1118
Main Authors: Bergman, Kristoffer, Engstrand, Thomas, Hilborn, Jöns, Ossipov, Dmitri, Piskounova, Sonya, Bowden, Tim
Format: Article
Language:English
Subjects:
Animals
Bone and Bones - diagnostic imaging
Bone and Bones - drug effects
Bone and Bones - pathology
bone morphogenetic protein
bone tissue engineering
Cell Death - drug effects
Cell Survival - drug effects
Chemistry
Choristoma - diagnostic imaging
Choristoma - pathology
Cross-Linking Reagents - pharmacology
Fibroblasts - cytology
Fibroblasts - drug effects
Fibroblasts - metabolism
Humans
hyaluronan
hydrogel
Hydrogels - pharmacology
injectable
Injections
Inorganic Chemistry
Kemi
Kinetics
NATURAL SCIENCES
NATURVETENSKAP
Oorganisk kemi
Osteogenesis - drug effects
Osteogenesis - physiology
Polymers - chemical synthesis
Polymers - pharmacology
polyvinyl alcohol
Prosthesis Implantation
Rats
Rats, Sprague-Dawley
X-Ray Microtomography
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Summary:Here we present a novel injectable hydrogel which forms a template for de novo formation of bone tissue. Hydrogel formation takes place in situ in less than 1 min by the cross‐linking of multifunctional hyaluronic acid and polyvinyl alcohol derivatives. Endogenous cells are recruited in vivo by incorporating bone morphogenetic protein‐2 (BMP‐2), a powerful promoter for osteogenic differentiation. The hydrogel was evaluated in vitro by performing a cell viability test and a release study and in vivo by a rat ectopic model. Examination by X‐ray, microcomputed tomography, and histology revealed a significant bone formation at the target site for gels containing BMP‐2, and a complete degradation was observed for gels without BMP‐2 four weeks after injection. There were no signs of inflammation or foreign body response in either group and we believe that this system has the potential as an off‐the‐shelf injectable to be used where bone tissue is needed. © 2008 Wiley Periodicals, Inc. J Biomed Mater Res, 2009
ISSN:1549-3296
1552-4965
1552-4965
DOI:10.1002/jbm.a.32289