Novel polyamine coating providing non-covalent deactivation and reversed electroosmotic flow of fused-silica capillaries for capillary electrophoresis

• Published in: Journal of Chromatography A 2003-06, Vol.1003 (1), p.217-221
• Main Authors: Hardenborg, Emilia, Zuberovic, Aida, Ullsten, Sara, Söderberg, Lennart, Heldin, Eva, Markides, Karin E.
• Format: Article
• Language: English
• Subjects: Adsorption; Analytical, structural and metabolic biochemistry; Biological and medical sciences; Cations; Electrophoresis, Capillary - instrumentation; Electrophoresis, Capillary - methods; Fundamental and applied biological sciences. Psychology; General aspects, investigation methods; Hydrogen-Ion Concentration; Isoelectric Point; Osmosis; Polyamines; Proteins; Proteins - isolation & purification; Silicon Dioxide; Static Electricity; Surface Properties
• ISSN: 0021-9673
• DOI: 10.1016/S0021-9673(03)00739-8

A new polycationic coating for use in capillary electrophoresis has been developed that enables chemical modification of fused-silica capillary surfaces for analysis of compounds like basic proteins. The cationic polyamine, containing short aliphatic blocks of combined 2 and 3-carbon length, was physically adsorbed onto the negatively charged fused-silica surface through ionic interaction by flushing the capillary with a polyamine solution, followed by a self-stabilization step. The polyamine coated capillaries generated an anodal electroosmotic flow that was independent of pH in the investigated range of pH 4–8. The capillary performance was demonstrated by fast separations of basic proteins with peak efficiencies in the range of 265 000–584 000 plates.