Emergence of fluoroquinolone-resistant clonal group A: clonal analysis of Norwegian and Russian E. coli isolates

• Published in: APMIS : acta pathologica, microbiologica et immunologica Scandinavica microbiologica et immunologica Scandinavica, 2010-08, Vol.118 (8), p.571-577
• Main Authors: STRAND, LINDA, JENKINS, ANDREW, GRUDE, NILS, ALLUM, ANNE-GRY, MYKLAND, HILDE-CHRISTINE, NOWROUZIAN, FOUROUGH L, KRISTIANSEN, BJøRN-ERIK
• Format: Article
• Language: English
• Subjects: analysis; Anti-Bacterial Agents; Anti-Bacterial Agents - pharmacology; Bacterial; Biological and medical sciences; CgA; classification; clonality; DNA fingerprinting; drug effects; Drug Resistance; Drug Resistance, Bacterial; E.coli; Escherichia coli; Escherichia coli - classification; Escherichia coli - drug effects; Escherichia coli - pathogenicity; Female; fluoroquinolone resistance; Fluoroquinolones; Fluoroquinolones - pharmacology; Fundamental and applied biological sciences. Psychology; Humans; Infectious diseases; Infectious Medicine; Infektionsmedicin; Intestines; Intestines - microbiology; Medical sciences; Microbiology; Microbiology in the medical area; Mikrobiologi inom det medicinska området; pathogenicity; pharmacology; phylogenetic group; Phylogeny; Ribotyping; Urinary Tract; Urinary Tract - microbiology; Virulence Factors; Virulence Factors - analysis
• ISSN: 0903-4641; 1600-0463; 1600-0463
• DOI: 10.1111/j.1600-0463.2010.02623.x

Strand L, Jenkins A, Grude N, Allum A-G, Mykland H-C, Nowrouzian FL, Kristiansen B-E. Emergence of fluoroquinolone-resistant clonal group A: clonal analysis of Norwegian and Russian E. coli isolates. APMIS 2010; 118: 571-77. We describe a study of urinary tract and intestinal isolates of Escherichia coli from Norway and Russia using automated ribotyping, single nucleotide polymorphism analysis for clonal group A (CgA) supplemented with phylogrouping, virulence gene profiling and resistance profiling. CgA comprised 19% of the Norwegian UTI isolates from 2001. Two highly multiresistant fluoroquinolone-resistant CgA isolates were found. Ribotypes clustered into four major and six minor groups (ribogroups). Fluoroquinolone-resistant isolates and phylogroups A and B1 were associated with ribogroup RA. Ribogroup RB predominated among Russian UTI isolates and was predominantly phylogroup A and depleted in P-fimbriae. Ribogroup RC predominated among Norwegian UTI isolates and was rich in virulence factors (S-fimbriae, haemagglutinin and haemolysin) and predominantly phylogroup B2 and D. Ribogroup RG was associated with CgA and predominantly phylogroup D. Ribogroups RD, RE and RF had too few members for statistical analysis. The correlation between ribotype and phylogenetic group was not as strong as reported in other studies.