Learning and Activity after Irradiation of the Young Mouse Brain Analyzed in Adulthood Using Unbiased Monitoring in a Home Cage Environment

Cranial radiotherapy during the treatment of pediatric malignancies may cause adverse late effects. It is important to find methods to assess the functional effects of ionizing radiation in animal models and to evaluate the possible ameliorating effects of preventive or reparative treatment strategi...

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Bibliographic Details
Published in:Radiation research 2011-03, Vol.175 (3), p.336-346
Main Authors: Karlsson, Niklas, Kalm, Marie, Nilsson, Marie K. L., Mallard, Carina, Björk-Eriksson, Thomas, Blomgren, Klas
Format: Article
Language:English
Subjects:
Animal models
Animals
Brain
Brain - physiology
Brain - radiation effects
Cancer and Oncology
Cancer och onkologi
Childhood
Conditioning (Psychology)
Conditioning (Psychology) - radiation effects
Educational activities
Fear
Fear - radiation effects
Freezing
Irradiation
Learning
Learning - radiation effects
Light
Male
MEDICAL AND HEALTH SCIENCES
MEDICIN OCH HÄLSOVETENSKAP
Memory
Mice
Motor Activity
Motor Activity - radiation effects
Physical
physiology
Radiation Dosage
radiation effects
Radiotherapy
REGULAR ARTICLES
Restraint
Restraint, Physical
Space life sciences
Time Factors
Water bottles
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Summary:Cranial radiotherapy during the treatment of pediatric malignancies may cause adverse late effects. It is important to find methods to assess the functional effects of ionizing radiation in animal models and to evaluate the possible ameliorating effects of preventive or reparative treatment strategies. We investigated the long-term effects of a single 8-Gy radiation dose to the brains of 14-day-old mice. Activity and learning were evaluated in adulthood using open field and trace fear conditioning (TFC). These established methods were compared with the novel IntelliCage platform, which enables unbiased analysis of both activity and learning over time in a home cage environment. Neither activity nor learning was changed after irradiation, as judged by the open field and TFC analyses. The IntelliCage, however, revealed both altered activity and learning impairment after irradiation. Place learning and reversal learning were both impaired in the IntelliCage 3 months after irradiation. These results indicate that activity and learning should be assessed using multiple methods and that unbiased analysis over time in a home cage environment may offer advantages in the detection of subtle radiation-induced effects on the young brain.
ISSN:0033-7587
1938-5404
1938-5404
DOI:10.1667/RR2231.1