Measuring absolute number concentrations of nanoparticles using single-particle tracking

Single-particle tracking (SPT) microscopy is increasingly used to characterize nanoparticulate systems. We introduce a concept for estimation of particle number concentration in Brownian particle dispersions using SPT based on a model for the trajectory length distribution of particles to estimate t...

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Bibliographic Details
Published in:Physical review. E, Statistical, nonlinear, and soft matter physics Statistical, nonlinear, and soft matter physics, 2011-09, Vol.84 (3 Pt 1), p.031920-031920, Article 031920
Main Authors: Röding, Magnus, Deschout, Hendrik, Braeckmans, Kevin, Rudemo, Mats
Format: Article
Language:English
Subjects:
Colloids - chemistry
Computer Simulation
delivery
Fysik
Models, Chemical
Models, Molecular
Nanoparticles - chemistry
Nanoparticles - ultrastructure
Particle Size
Physical Sciences
size
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Summary:Single-particle tracking (SPT) microscopy is increasingly used to characterize nanoparticulate systems. We introduce a concept for estimation of particle number concentration in Brownian particle dispersions using SPT based on a model for the trajectory length distribution of particles to estimate the detection region volume. The resulting method is independent of precalibration reference measurements, and robust with respect to image processing settings. Experimentally estimated concentrations of different dilutions of 0.19- and 0.52-μm polymer nanospheres are in excellent agreement with estimates computed from the concentrations of the stock solutions.
ISSN:1539-3755
1550-2376
1550-2376
DOI:10.1103/PhysRevE.84.031920