Plasmophore sensitized imaging of ammonia release from biological tissues using optodes

• Published in: Analytica chimica acta 2011-10, Vol.704 (1), p.139-145
• Main Authors: Strömberg, Niklas, Hakonen, Aron
• Format: Article
• Language: English
• Subjects: Ammonia; Ammonia - analysis; Analytical chemistry; Animals; Artificial Organs; Benzopyrans - chemistry; Biological; Biosensing Techniques - instrumentation; Biosensing Techniques - methods; Body Fluids - chemistry; Chemical Sciences; Chemistry; Chemistry Techniques, Analytical; Detection; Exact sciences and technology; Fluorescence; Fluorosensor; Gases - analysis; General, instrumentation; Humans; Hydrogels - chemistry; Imaging; Indoles - chemistry; Kemi; Limit of Detection; Macrolides - chemistry; Membranes, Artificial; Molecular Imaging - instrumentation; Molecular Imaging - methods; Muscle, Skeletal - chemistry; Muscle, Skeletal - metabolism; Muscles; Nanomaterials; Nanoparticles; Nanostructure; Nose; Optode; Plasmonics; Sensors; Spectrometric and optical methods; Swine; Tissue Engineering - methods; Tongue
• ISSN: 0003-2670; 1873-4324; 1873-4324
• DOI: 10.1016/j.aca.2011.08.019

[Display omitted] ► A plasmophore sensitized optode for imaging ammonia (NH 3) concentrations in muscle tissues was developed. ► Ammonia concentrations ranging from 10 nM and upwards can be quantified reversibly with an optical resolution of 127 μm. ► The general sensing scheme offers new possibilities for the development of artificial optical noses and tongues. A plasmophore sensitized optode was developed for imaging ammonia (NH 3) concentrations in muscle tissues. The developed ammonia sensor and an equivalent non plasmophore version of the sensor were tested side by side to compare their limit of detection, dynamic range, reversibility and overall imaging quality. Bio-degradation patterns of ammonia release from lean porcine skeletal muscle were studied over a period of 11 days. We demonstrate that ammonia concentrations ranging from 10 nM can be quantified reversibly with an optical resolution of 127 μm in a sample area of 25 mm × 35 mm. The plasmophore ammonia optode showed improved reversibility, less false pixels and a 2 nM ammonia detection limit compared to 200 nM for the non-plasmophore sensor. Main principles of the sensing mechanism include ammonia transfer over a gas permeable film, ammonia protonation, nonactin facilitated merocyanine-ammonium coextraction and plasmophore enhancement. The vast signal improvement is suggested to rely on solvatochroism, nanoparticle scattering and plasmonic interactions that are utilized constructively in a fluorescence ratio. In addition to fundamental medicinal and biological research applications in tissue physiology, reversible ammonia quantification will be possible for a majority of demanding imaging and non imaging applications such as monitoring of low ammonia background concentrations in air and non-invasive medicinal diagnosis through medical breath or saliva analysis. The nanoparticle doped sensor constitutes a highly competitive technique for ammonia sensing in complex matrixes and the general sensing scheme offers new possibilities for the development of artificial optical noses and tongues.