Targeting the glycoproteome

Despite numerous original publications describing the structural complexity of N- and O-linked glycans on glycoproteins, only very few answer the basic question of which particular glycans are linked to which amino acid residues along the polypeptide chain. Such structural information is of fundamen...

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Bibliographic Details
Published in:Glycoconjugate journal 2013-02, Vol.30 (2), p.119-136
Main Authors: Nilsson, Jonas, Halim, Adnan, Grahn, Ammi, Larson, Göran
Format: Article
Language:English
Subjects:
Annan klinisk medicin
Annan medicinsk grundvetenskap
Biochemistry
Biomedical and Life Sciences
Cells, Cultured
Chromatography
Chromatography, Liquid - methods
Clinical Laboratory Medicine
Glycopeptides - analysis
Glycopeptides - chemistry
Glycoproteins
Glycoproteins - analysis
Glycoproteins - chemistry
Glycosylation
Humans
Hydrophobic and Hydrophilic Interactions
Klinisk laboratoriemedicin
Life Sciences
Mass Spectrometry
Models, Biological
Other Basic Medicine
Other Clinical Medicine
Pathology
Peptides
Proteome - analysis
Proteome - chemistry
Proteomics
Structural Biology
Strukturbiologi
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Summary:Despite numerous original publications describing the structural complexity of N- and O-linked glycans on glycoproteins, only very few answer the basic question of which particular glycans are linked to which amino acid residues along the polypeptide chain. Such structural information is of fundamental importance for understanding the biological roles of complex glycosylations as well as deciphering their non-template driven biosynthesis. This review focuses on presenting and commenting on recent strategies, specifically aimed at identifying the glycoproteome of cultured cells and biological samples, using targeted and global enrichment procedures and utilizing the high resolution power, high through-put capacity and complementary fragmentation techniques of tandem mass spectrometry. The goal is to give an update of this emerging field of protein and glyco-sciences and suggest routes to bridge the data gap between the two aspects of glycoprotein characteristics, i.e. glycan structures and their attachment sites.
ISSN:0282-0080
1573-4986
1573-4986
DOI:10.1007/s10719-012-9438-6