Heat Stabilization of Blood Spot Samples for Determination of Metabolically Unstable Drug Compounds

Background: Sample stability is critical for accurate analysis of drug compounds in biosamples. The use of additives to eradicate the enzymatic activity causing loss of these analytes has its limitations. Results: A novel technique for sample stabilization by rapid, high-temperature heating was used...

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Published in:Bioanalysis 2013-01, Vol.5 (1), p.31-39
Main Authors: Blessborn, Daniel, Sköld, Karl, Zeeberg, David, Kaewkhao, Karnrawee, Sköld, Olof, Ahnoff, Martin
Format: Article
Language:English
Subjects:
amodiaquine
assay
biological samples
Chemical Sciences
chloroquine
Kemi
liquid-chromatography
mass-spectrometry
oseltamivir
plasma
stability
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cited_by cdi_FETCH-LOGICAL-c416t-bd2910ccc529b3a112f99473849d90c735157042dad507ce18c158b51a5d01933
cites cdi_FETCH-LOGICAL-c416t-bd2910ccc529b3a112f99473849d90c735157042dad507ce18c158b51a5d01933
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creator Blessborn, Daniel
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Ahnoff, Martin
description Background: Sample stability is critical for accurate analysis of drug compounds in biosamples. The use of additives to eradicate the enzymatic activity causing loss of these analytes has its limitations. Results: A novel technique for sample stabilization by rapid, high-temperature heating was used. The stability of six commercial drugs in blood and blood spots was investigated under various conditions with or without heat stabilization at 95 degrees C. Oseltamivir, cefotaxime and ribavirin were successfully stabilized by heating whereas significant losses were seen in unheated samples. Amodiaquine was stable with and without heating. Artemether and dihydroartemisinin were found to be very heat sensitive and began to decompose even at 60 degrees C. Conclusion: Heat stabilization is a viable technique to maintain analytes in blood spot samples, without the use of chemical additives, by stopping the enzymatic activity that causes sample degradation.
doi_str_mv 10.4155/bio.12.294
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subjects amodiaquine
assay
biological samples
Chemical Sciences
chloroquine
Kemi
liquid-chromatography
mass-spectrometry
oseltamivir
plasma
stability
title Heat Stabilization of Blood Spot Samples for Determination of Metabolically Unstable Drug Compounds
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