Site-specific O-Glycosylation on the MUC2 Mucin Protein Inhibits Cleavage by the Porphyromonas gingivalis Secreted Cysteine Protease (RgpB)

• Published in: The Journal of biological chemistry 2013-05, Vol.288 (20), p.14636-14646
• Main Authors: van der Post, Sjoerd, Subramani, Durai B., Bäckström, Malin, Johansson, Malin E.V., Vester-Christensen, Malene B., Mandel, Ulla, Bennett, Eric P., Clausen, Henrik, Dahlén, Gunnar, Sroka, Aneta, Potempa, Jan, Hansson, Gunnar C.
• Format: Article
• Language: English
• Subjects: Adhesins; Adhesins, Bacterial - metabolism; Amino Acid Sequence; Animals; Bacterial; Bacterial Pathogenesis; Basic Medicine; CHO Cells; Chromatography; Colitis; Colitis - microbiology; Colon; Colon - metabolism; Cricetinae; Cysteine Endopeptidases; Cysteine Endopeptidases - metabolism; enzymology; Epithelium; Epithelium - metabolism; Gingipain Cysteine Endopeptidases; Glycobiology and Extracellular Matrices; Glycoprotein Biosynthesis; Glycosylation; Glycosyltransferases; Humans; Mass Spectrometry; Medicinska och farmaceutiska grundvetenskaper; metabolism; microbiology; Molecular Sequence Data; Mucin-2; Mucin-2 - metabolism; Mucins; Mucus; Porphyromonas gingivalis; Porphyromonas gingivalis - enzymology
• ISSN: 0021-9258; 1083-351X; 1083-351X
• DOI: 10.1074/jbc.M113.459479

The colonic epithelial surface is protected by an inner mucus layer that the commensal microflora cannot penetrate. We previously demonstrated that Entamoeba histolytica secretes a protease capable of dissolving this layer that is required for parasite penetration. Here, we asked whether there are bacteria that can secrete similar proteases. We screened bacterial culture supernatants for such activity using recombinant fragments of the MUC2 mucin, the major structural component, and the only gel-forming mucin in the colonic mucus. MUC2 has two central heavily O-glycosylated mucin domains that are protease-resistant and has cysteine-rich N and C termini responsible for polymerization. Culture supernatants of Porphyromonas gingivalis, a bacterium that secretes proteases responsible for periodontitis, cleaved the MUC2 C-terminal region, whereas the N-terminal region was unaffected. The active enzyme was isolated and identified as Arg-gingipain B (RgpB). Two cleavage sites were localized to IR↓TT and NR↓QA. IR↓TT cleavage will disrupt the MUC2 polymers. Because this site has two potential O-glycosylation sites, we tested whether recombinant GalNAc-transferases (GalNAc-Ts) could glycosylate a synthetic peptide covering the IRTT sequence. Only GalNAc-T3 was able to glycosylate the second Thr in IRTT, rendering the sequence resistant to cleavage by RgpB. Furthermore, when GalNAc-T3 was expressed in CHO cells expressing the MUC2 C terminus, the second threonine was glycosylated, and the protein became resistant to RgpB cleavage. These findings suggest that bacteria can produce proteases capable of dissolving the inner protective mucus layer by specific cleavages in the MUC2 mucin and that this cleavage can be modulated by site-specific O-glycosylation. Background: MUC2 polymers form the mucus layer of colon that separates luminal bacteria from the epithelium. Results:P. gingivalis secrets a protease that cleaves the MUC2 mucin, a cleavage modulated by O-glycosylation. Conclusion: Bacteria can disrupt the MUC2 polymer via proteolytic cleavage. However, O-glycosylation can inhibit this process. Significance: Bacteria can dissolve the protective inner mucus layer, potentially triggering colitis.