Quantitative detection of epidermal growth factor and interleukin-8 in whole saliva of healthy individuals

• Published in: Journal of immunological methods 2014-06, Vol.408, p.46-51
• Main Authors: Dafar, Amal, Rico, Paula, Işık, Ayşegül, Jontell, Mats, Çevik-Aras, Hülya
• Format: Article
• Language: English
• Subjects: Adult; Age Factors; Aged; Biomarkers - analysis; Detergents - chemistry; Eating; EGF; ELISA; Enzyme-Linked Immunosorbent Assay - standards; Epidermal Growth Factor - analysis; Female; Healthy Volunteers; Humans; IL-8; Immunologi inom det medicinska området; Immunology in the medical area; inflammatory markers; Interleukin-8 - analysis; Male; Middle Aged; Oral diseases; Predictive Value of Tests; Protease Inhibitors - chemistry; Protein biomarkers; saliva; Saliva - chemistry; Salivary diagnostics; Salivation; SDS; Sex Factors; Sodium Dodecyl Sulfate - chemistry; Specimen Handling; Young Adult
• ISSN: 0022-1759; 1872-7905
• DOI: 10.1016/j.jim.2014.04.013

This study aims to create consensus concerning the use of a methodology by which the handling of saliva is standardized and quantitative detection of IL-8 and EGF in whole saliva is achieved. Our study involves evaluating the extent to which the pre-treatment of saliva samples with an anionic detergent – sodium dodecyl sulphate (SDS) – improved detection levels for IL-8 and EGF. Whole saliva samples (n=28) were collected from healthy individuals and a protease inhibitor cocktail was added immediately. They were treated with either SDS or PBS for 20min and were then applied to a sandwich ELISA. Saliva is a complex viscous fluid that requires degrading before the analysis of salivary biomarkers. We found that pre-treatment of samples with SDS significantly increased the detection levels for both EGF (293%) and IL-8 (346%) when compared with PBS-treated pairs (⁎⁎⁎P