Cortical astrogliosis and increased perivascular aquaporin-4 in idiopathic intracranial hypertension

• Published in: Brain research 2016-08, Vol.1644, p.161-175
• Main Authors: Eide, Per Kristian, Eidsvaag, Vigdis Andersen, Nagelhus, Erlend A, Hansson, Hans-Arne
• Format: Article
• Language: English
• Subjects: Adult; Aquaporin 4 - metabolism; Aquaporin-4; Astrocytes - metabolism; Astrocytes - pathology; Astrogliosis; benign; Benign intracranial hypertension; body-mass index; brain; Cerebral Cortex - metabolism; Cerebral Cortex - pathology; csf opening pressure; dark neuron; Female; Gliosis - complications; Humans; Interstitial fluid disturbance; Male; Middle Aged; Neurologi; Neurology; Neurons - metabolism; Neurons - pathology; Neurosciences & Neurology; normal-pressure hydrocephalus; obesity; optic-nerve; Prospective Studies; Pseudotumor cerebri; Pseudotumor Cerebri - complications; Pseudotumor Cerebri - metabolism; Pseudotumor Cerebri - pathology; Pulsatile intracranial pressure; reference interval; Young Adult
• ISSN: 0006-8993; 1872-6240
• DOI: 10.1016/j.brainres.2016.05.024

Abstract The syndrome idiopathic intracranial hypertension (IIH) includes symptoms and signs of raised intracranial pressure (ICP) and impaired vision, usually in overweight persons. The pathogenesis is unknown. In the present prospective observational study, we characterized the histopathological changes in biopsies from the frontal brain cortical parenchyma obtained from 18 IIH patients. Reference specimens were sampled from 13 patients who underwent brain surgery for epilepsy, tumors or acute vascular diseases. Overnight ICP monitoring revealed abnormal intracranial pressure wave amplitudes in 14/18 IIH patients, who underwent shunt surgery and all responded favorably. A remarkable histopathological observation in IIH patients was patchy astrogliosis defined as clusters of hypertrophic astrocytes enclosing a nest of nerve cells. Distinct astrocyte domains (i.e. no overlap between astrocyte processes) were lacking in most IIH biopsy specimens, in contrast to their prevalence in reference specimens. Evidence of astrogliosis in IIH was accompanied with significantly increased aquaporin-4 (AQP4) immunoreactivity over perivascular astrocytic endfeet, compared to the reference specimens, measured with densitometry. Scattered CD68 immunoreactive cells (activated microglia and macrophages) were recognized, indicative of some inflammation. No apoptotic cells were demonstrable. We conclude that the patchy astrogliosis is a major finding in patients with IIH. We propose that the astrogliosis impairs intracranial pressure-volume reserve capacity, i.e. intracranial compliance, and contributes to the IIH by restricting the outflow of fluid from the cranium. The increased perivascular AQP4 in IIH may represent a compensatory mechanism to enhance brain fluid drainage.