Molecular profiling of mucosal tissue associated microbiota in patients manifesting acute exacerbations and remission stage of ulcerative colitis

• Published in: World journal of microbiology & biotechnology 2018-06, Vol.34 (6), p.76-13, Article 76
• Main Authors: Walujkar, Sandeep A., Kumbhare, Shreyas V., Marathe, Nachiket P., Patangia, Dhrati V., Lawate, Parimal S., Bharadwaj, Renu S., Shouche, Yogesh S.
• Format: Article
• Language: English
• Subjects: Achromobacter; Applied Microbiology; Bacteria; Biochemistry; Biomedical and Life Sciences; Biopsy; Biotechnology; Biotechnology & Applied Microbiology; chain fatty-acids; Colon; Communities; diversity; Dysbacteriosis; Dysbiosis; Environmental Engineering/Biotechnology; faecalibacterium-prausnitzii; Gene sequencing; gut microbiota; Gut microflora; health; Infectious Medicine; Infektionsmedicin; Inflammatory bowel disease; intestinal microbiota; Intestinal microflora; Intestine; Life Sciences; Microbial activity; Microbiology; Microbiota; Microorganisms; Molecular chains; Mucosa; Mucosal tissue inflammation; Original Paper; Pathogenesis; Patients; quality-of-life; Rank tests; Remission; rRNA 16S; Stenotrophomonas; stenotrophomonas-maltophilia; Ulcerative colitis
• ISSN: 0959-3993; 1573-0972
• DOI: 10.1007/s11274-018-2449-0

Dysbiosis of intestinal microflora has been postulated in ulcerative colitis (UC), which is characterized by imbalance of mucosal tissue associated bacterial communities. However, the specific changes in mucosal microflora during different stages of UC are still unknown. The aim of the current study was to investigate the changes in mucosal tissue associated microbiota during acute exacerbations and remission stages of UC. The mucosal microbiota associated with colon biopsy of 12 patients suffering from UC (exacerbated stage) and the follow-up samples from the same patients (remission stage) as well as non-IBD subjects was studied using 16S rRNA gene-based sequencing and quantitative PCR. The total bacterial count in patients suffering from exacerbated phase of UC was observed to be two fold lower compared to that of the non-IBD subjects ( p  = 0.0049, Wilcox on matched-pairs signed rank tests). Bacterial genera including Stenotrophomonas, Parabacteroides, Elizabethkingia, Pseudomonas, Micrococcus, Ochrobactrum and Achromobacter were significantly higher in abundance during exacerbated phase of UC as compared to remission phase. The alterations in bacterial diversity with an increase in the abnormal microbial communities signify the extent of dysbiosis in mucosal microbiota in patients suffering from UC. Our study helps in identifying the specific genera dominating the microbiota during the disease and thus lays a basis for further investigation of the possible role of these bacteria in pathogenesis of UC.