XLSY: Extra‐Large NMR Spectroscopy

NMR studies of intrinsically disordered proteins and other complex biomolecular systems require spectra with the highest resolution and dimensionality. An efficient approach, extra‐large NMR spectroscopy, is presented for experimental data collection, reconstruction, and handling of very large NMR s...

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Bibliographic Details
Published in:Angewandte Chemie International Edition 2018-10, Vol.57 (43), p.14043-14045
Main Authors: Pustovalova, Yulia, Mayzel, Maxim, Orekhov, Vladislav Yu
Format: Article
Language:English
Subjects:
Algorithms
alpha-Synuclein - chemistry
Biochemistry and Molecular Biology
Biofysik
Biokemi och molekylärbiologi
Biophysics
Communication
Communications
Data collection
intrinsically disordered protein
Intrinsically Disordered Proteins - chemistry
Magnetic resonance spectroscopy
NMR spectroscopy
non-uniform sampling
Nuclear Magnetic Resonance, Biomolecular - methods
Protein Conformation
Proteins
Reconstruction
Spectroscopy
Spectrum analysis
Statistical analysis
Structural Biology
Strukturbiologi
Synuclein
XLSY
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Summary:NMR studies of intrinsically disordered proteins and other complex biomolecular systems require spectra with the highest resolution and dimensionality. An efficient approach, extra‐large NMR spectroscopy, is presented for experimental data collection, reconstruction, and handling of very large NMR spectra by a combination of the radial and non‐uniform sampling, a new processing algorithm, and rigorous statistical validation. We demonstrate the first high‐quality reconstruction of a full seven‐dimensional HNCOCACONH and two five‐dimensional HACACONH and HN(CA)CONH experiments for a representative intrinsically disordered protein α‐synuclein. XLSY will significantly enhance the NMR toolbox in challenging biomolecular studies. XLSY, an efficient approach for acquisition, reconstruction, and analysis of very large spectra, is presented. High‐quality reconstruction of a full seven‐dimensional and two five‐dimensional experiments for a representative intrinsically disordered protein alpha‐synuclein is demonstrated. XLSY will significantly enhance the NMR toolbox in challenging biomolecular studies.
ISSN:1433-7851
1521-3773
1521-3773
DOI:10.1002/anie.201806144