Distinct glycosylation in membrane proteins within neonatal versus adult myocardial tissue

• Published in: Matrix biology 2020-01, Vol.85-86, p.173-188
• Main Authors: Contessotto, Paolo, Ellis, Bradley W., Jin, Chunsheng, Karlsson, Niclas G., Zorlutuna, Pinar, Kilcoyne, Michelle, Pandit, Abhay
• Format: Article
• Language: English
• Subjects: Aging; Animals; Animals, Newborn; Biological Sciences; Biologiska vetenskaper; Carbohydrates; Cell surface; Extracellular matrix; Fucose - metabolism; Gene Expression Regulation, Developmental; Glycobiology; Glycomics; Glycosylation; Heart; Heart regeneration; Heart Ventricles - metabolism; Intracellular signalling; Lectin microarrays; Lectins - metabolism; Mannose; Mass Spectrometry; Maturation; Membrane Glycoproteins - metabolism; Membrane proteins; Mucin; Mucins - metabolism; Myocardial infarction; N-Acetylneuraminic acid; N-Acetylneuraminic Acid - chemistry; N-glycans; Neonatal; Neonates; Neuraminic Acids - chemistry; Oligosaccharides - metabolism; Polysaccharides; Rats; Rats, Sprague-Dawley; Tissue Array Analysis - methods; Ventricle
• ISSN: 0945-053X; 1569-1802
• DOI: 10.1016/j.matbio.2019.05.001

Mammalian hearts have regenerative potential restricted to early neonatal stage and lost within seven days after birth. Carbohydrates exclusive to cardiac neonatal tissue may be key regulators of regenerative potential. Although cell surface and extracellular matrix glycosylation are known modulators of tissue and cellular function and development, variation in cardiac glycosylation from neonatal tissue to maturation has not been fully examined. In this study, glycosylation of the adult rat cardiac ventricle showed no variability between the two strains analysed, nor were there any differences between the glycosylation of the right or left ventricle using lectin histochemistry and microarray profiling. However, in the Sprague-Dawley strain, neonatal cardiac glycosylation in the left ventricle differed from adult tissues using mass spectrometric analysis, showing a higher expression of high mannose structures and lower expression of complex N-linked glycans in the three-day-old neonatal tissue. Man6GlcNAc2 was identified as the main high mannose N-linked structure that was decreased in adult while higher expression of sialylated N-linked glycans and lower core fucosylation for complex structures were associated with ageing. The occurrence of mucin core type 2 O-linked glycans was reduced in adult and one sulfated core type 2 O-linked structure was identified in neonatal tissue. Interestingly, O-linked glycans from mature tissue contained both N-acetylneuraminic acid (Neu5Ac) and N-glycolylneuraminic acid (Neu5Gc), while all sialylated N-linked glycans detected contained only Neu5Ac. As glycans are associated with intracellular communication, the specific neonatal structures found may indicate a role for glycosylation in the neonatal associated regenerative capacity of the mammalian heart. New strategies targeting tissue glycosylation could be a key contributor to achieve an effective regeneration of the mammalian heart in pathological scenarios such as myocardial infarction. Schematics showing the experimental flow of the study from lectin histochemistry to PGC LC-ESI-MS/MS analyses (spectra) and lectin microarrays (heat-maps) to identify the glycan structures present in adult and neonatal myocardial tissues. [Display omitted]