Comparative analysis of obesity-related cardiometabolic and renal biomarkers in human plasma and serum

The search for biomarkers associated with obesity-related diseases is ongoing, but it is not clear whether plasma and serum can be used interchangeably in this process. Here we used high-throughput screening to analyze 358 proteins and 76 lipids, selected because of their relevance to obesity-associ...

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Bibliographic Details
Published in:Scientific reports 2019-10, Vol.9 (1), p.15385-15385, Article 15385
Main Authors: Rajan, Meenu Rohini, Sotak, Matus, Barrenäs, Fredrik, Shen, Tong, Borkowski, Kamil, Ashton, Nicholas J., Biörserud, Christina, Lindahl, Tomas L., Ramström, Sofia, Schöll, Michael, Lindahl, Per, Fiehn, Oliver, Newman, John W., Perkins, Rosie, Wallenius, Ville, Lange, Stephan, Börgeson, Emma
Format: Article
Language:English
Subjects:
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Adult
Basic Medicine
Biomarkers
Biomarkers - blood
cardiometabolic disease
Cardiovascular diseases
Cholesterol
Clinical Medicine
Comparative analysis
Female
High-throughput screening
Homeostasis
Humanities and Social Sciences
Humans
Kidney Diseases - blood
Kidney Diseases - complications
Klinisk medicin
Lipids
Male
Medicinska grundvetenskaper
Metabolic Syndrome - blood
Metabolic Syndrome - complications
Middle Aged
multidisciplinary
Obesity
Obesity - blood
Obesity - complications
Olink
Plasminogen Activator Inhibitor 1 - blood
Plasminogen Activator Inhibitor 1 - standards
Proprotein Convertase 9 - blood
Proprotein Convertase 9 - standards
Science
Science (multidisciplinary)
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Summary:The search for biomarkers associated with obesity-related diseases is ongoing, but it is not clear whether plasma and serum can be used interchangeably in this process. Here we used high-throughput screening to analyze 358 proteins and 76 lipids, selected because of their relevance to obesity-associated diseases, in plasma and serum from age- and sex-matched lean and obese humans. Most of the proteins/lipids had similar concentrations in plasma and serum, but a subset showed significant differences. Notably, a key marker of cardiovascular disease PAI-1 showed a difference in concentration between the obese and lean groups only in plasma. Furthermore, some biomarkers showed poor correlations between plasma and serum, including PCSK9, an important regulator of cholesterol homeostasis. Collectively, our results show that the choice of biofluid may impact study outcome when screening for obesity-related biomarkers and we identify several markers where this will be the case.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-019-51673-0