Effects of C-Peptide Replacement Therapy on Bone Microarchitecture Parameters in Streptozotocin-Diabetic Rats

C-peptide therapy protects against diabetic micro- and macrovascular damages and neuropatic complications. However, to date, the role of C-peptide in preventing diabetes-related bone loss has not been investigated. Our aim was to evaluate if C-peptide infusion improves bone quality in diabetic rats....

Full description

Saved in:
Bibliographic Details
Published in:Calcified tissue international 2020-09, Vol.107 (3), p.266-280
Main Authors: Maurotti, Samantha, Russo, Cristina, Musolino, Vincenzo, Nucera, Saverio, Gliozzi, Micaela, Scicchitano, Miriam, Bosco, Francesca, Morittu, Valeria Maria, Ragusa, Monica, Mazza, Elisa, Pujia, Roberta, Gazzaruso, Carmine, Britti, Domenico, Valenti, Maria Teresa, Deiana, Michela, Romeo, Stefano, Giannini, Sandro, Dalle Carbonare, Luca, Mollace, Vincenzo, Pujia, Arturo, Montalcini, Tiziana
Format: Article
Language:English
Subjects:
Biochemistry
Biomedical and Life Sciences
Bone loss
Calcium
Cancellous bone
Cardiac and Cardiovascular Systems
Cell Biology
Computed tomography
Diabetes
Diabetes mellitus
Endocrinology
Kardiologi
Life Sciences
Original Research
Orthopedics
Osteoporosis
Peptides
Rodents
Streptozocin
Tibia
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:C-peptide therapy protects against diabetic micro- and macrovascular damages and neuropatic complications. However, to date, the role of C-peptide in preventing diabetes-related bone loss has not been investigated. Our aim was to evaluate if C-peptide infusion improves bone quality in diabetic rats. Twenty-three male Wistar rats were randomly divided into three groups: normal control group; sham diabetic control group; diabetic plus C-peptide group. Diabetes was induced by streptozotocin injection and C-peptide was delivered subcutaneously for 6 weeks. We performed micro-CT and histological testing to assess several trabecular microarchitectural parameters. At the end, diabetic plus C-peptide rats had a higher serum C-peptide ( p  = 0.02) and calcium ( p  = 0.04) levels and tibia weight ( p  = 0.02) than the diabetic control group. The diabetic plus C-peptide group showed a higher trabecular thickness and cross-sectional thickness than the diabetic control group ( p  = 0.01 and p  = 0.03). Both the normal control and diabetic plus C-peptide groups had more Runx-2 and PLIN1 positive cells in comparison with the diabetic control group ( p  = 0.045 and p  = 0.034). Diabetic rats receiving C-peptide had higher quality of trabecular bone than diabetic rats not receiving this treatment. If confirmed, C-peptide could have a role in improving bone quality in diabetes.
ISSN:0171-967X
1432-0827
1432-0827
DOI:10.1007/s00223-020-00716-0