Elevated expression of stemness genes in adipose-derived mesenchymal stem cells cultured on fibrin scaffold

In regenerative medicine, MSCs need to be pluripotent for better results. In this study, the effect of fibrin scaffold on expression of stemness genes was examined. Adipose-derived MSCs were cultured in tissue culture plates (2D) and 3-dimensional (3D) fibrin scaffolds. The effect of fibrin scaffold...

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Bibliographic Details
Published in:Journal of biosciences 2020-12, Vol.45 (1), Article 81
Main Authors: Mousavifard, Arezoo, Pourazizi Najafabadi, Elahe, Akhavan Rahnama, Mahshid, Anbarlou, Azadeh, Atashi, Amir
Format: Article
Language:English
Subjects:
Adipose Tissue - cytology
Biomedical and Life Sciences
Biomedicine
bone-marrow
Cell Adhesion
Cell Biology
Cell culture
Cell Culture Techniques
Cell Proliferation - physiology
Cell Survival - physiology
Cell therapy
Clinical Medicine
DNA
Evaluation
Fibrin
Fibrin scaffold
Flow cytometry
Gene expression
Gene Expression Regulation - physiology
Genes
Humans
Instruments
Klinisk medicin
Life Sciences
Life Sciences & Biomedicine - Other Topics
maintenance
Medical sciences
Mesenchymal stem cells
Mesenchymal Stem Cells - physiology
Microbiology
Microscopy, Electron, Scanning
Nanog
Nanog Homeobox Protein - genetics
Nanog Homeobox Protein - metabolism
Nanostructures
Nucleotide sequence
Oct-4 protein
OCT4
PCR
Plant Sciences
Pluripotency
Proliferation
Proteins
Regenerative medicine
Regulators
Scaffolds
SOX2
Stem cells
stemness
Surface Properties
Tissue culture
Tissue engineering
Tissue Scaffolds
Zoology
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Summary:In regenerative medicine, MSCs need to be pluripotent for better results. In this study, the effect of fibrin scaffold on expression of stemness genes was examined. Adipose-derived MSCs were cultured in tissue culture plates (2D) and 3-dimensional (3D) fibrin scaffolds. The effect of fibrin scaffold on proliferation of adipose-derived MSCs was evaluated by MTT assay. The expression of stemness genes ( OCT4 and SOX2 ) were evaluated by qRT-PCR, and flow cytometry was done for Nanog protein level. Cultured MSCs on fibrin scaffold were able to proliferate according to data obtained by MTT assay. Expression of OCT4 and SOX2 had a significant increase in cells were cultured in 3D condition compared to 2D condition (P 
ISSN:0250-5991
0973-7138
DOI:10.1007/s12038-020-00050-5