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Elevated expression of stemness genes in adipose-derived mesenchymal stem cells cultured on fibrin scaffold
In regenerative medicine, MSCs need to be pluripotent for better results. In this study, the effect of fibrin scaffold on expression of stemness genes was examined. Adipose-derived MSCs were cultured in tissue culture plates (2D) and 3-dimensional (3D) fibrin scaffolds. The effect of fibrin scaffold...
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| Published in: | Journal of biosciences 2020-12, Vol.45 (1), Article 81 |
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| cites | cdi_FETCH-LOGICAL-c441t-9d542abaafb3ef3553bdbcb2dfcc970a114a840e17ede4fe7749789fb7bd07943 |
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| container_title | Journal of biosciences |
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| creator | Mousavifard, Arezoo Pourazizi Najafabadi, Elahe Akhavan Rahnama, Mahshid Anbarlou, Azadeh Atashi, Amir |
| description | In regenerative medicine, MSCs need to be pluripotent for better results. In this study, the effect of fibrin scaffold on expression of stemness genes was examined. Adipose-derived MSCs were cultured in tissue culture plates (2D) and 3-dimensional (3D) fibrin scaffolds. The effect of fibrin scaffold on proliferation of adipose-derived MSCs was evaluated by MTT assay. The expression of stemness genes (
OCT4
and
SOX2
) were evaluated by qRT-PCR, and flow cytometry was done for Nanog protein level. Cultured MSCs on fibrin scaffold were able to proliferate according to data obtained by MTT assay. Expression of
OCT4
and
SOX2
had a significant increase in cells were cultured in 3D condition compared to 2D condition (P |
| doi_str_mv | 10.1007/s12038-020-00050-5 |
| format | article |
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OCT4
and
SOX2
) were evaluated by qRT-PCR, and flow cytometry was done for Nanog protein level. Cultured MSCs on fibrin scaffold were able to proliferate according to data obtained by MTT assay. Expression of
OCT4
and
SOX2
had a significant increase in cells were cultured in 3D condition compared to 2D condition (P < 0.05). Also, increased expression of Nanog protein in 3D culture was observed (P < 0.05).
OCT4
and
SOX2
in 3D condition increased two-fold and three-fold respectively in 2D and 3D conditions. Moreover, expression of Nanog increased 30% more than in 2D condition. Evaluation of important pluripotency regulators such as OCT4, SOX2, and Nanog showed that fibrin scaffolds are useful instruments to maintain stemness of MSCs, which is essential in field of stem cell therapy and regenerative medicine.</description><identifier>ISSN: 0250-5991</identifier><identifier>EISSN: 0973-7138</identifier><identifier>DOI: 10.1007/s12038-020-00050-5</identifier><identifier>PMID: 32554906</identifier><language>eng</language><publisher>New Delhi: Springer India</publisher><subject>Adipose Tissue - cytology ; Biomedical and Life Sciences ; Biomedicine ; bone-marrow ; Cell Adhesion ; Cell Biology ; Cell culture ; Cell Culture Techniques ; Cell Proliferation - physiology ; Cell Survival - physiology ; Cell therapy ; Clinical Medicine ; DNA ; Evaluation ; Fibrin ; Fibrin scaffold ; Flow cytometry ; Gene expression ; Gene Expression Regulation - physiology ; Genes ; Humans ; Instruments ; Klinisk medicin ; Life Sciences ; Life Sciences & Biomedicine - Other Topics ; maintenance ; Medical sciences ; Mesenchymal stem cells ; Mesenchymal Stem Cells - physiology ; Microbiology ; Microscopy, Electron, Scanning ; Nanog ; Nanog Homeobox Protein - genetics ; Nanog Homeobox Protein - metabolism ; Nanostructures ; Nucleotide sequence ; Oct-4 protein ; OCT4 ; PCR ; Plant Sciences ; Pluripotency ; Proliferation ; Proteins ; Regenerative medicine ; Regulators ; Scaffolds ; SOX2 ; Stem cells ; stemness ; Surface Properties ; Tissue culture ; Tissue engineering ; Tissue Scaffolds ; Zoology</subject><ispartof>Journal of biosciences, 2020-12, Vol.45 (1), Article 81</ispartof><rights>Indian Academy of Sciences 2020</rights><rights>Indian Academy of Sciences 2020.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c441t-9d542abaafb3ef3553bdbcb2dfcc970a114a840e17ede4fe7749789fb7bd07943</citedby><cites>FETCH-LOGICAL-c441t-9d542abaafb3ef3553bdbcb2dfcc970a114a840e17ede4fe7749789fb7bd07943</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32554906$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://gup.ub.gu.se/publication/294849$$DView record from Swedish Publication Index$$Hfree_for_read</backlink></links><search><creatorcontrib>Mousavifard, Arezoo</creatorcontrib><creatorcontrib>Pourazizi Najafabadi, Elahe</creatorcontrib><creatorcontrib>Akhavan Rahnama, Mahshid</creatorcontrib><creatorcontrib>Anbarlou, Azadeh</creatorcontrib><creatorcontrib>Atashi, Amir</creatorcontrib><title>Elevated expression of stemness genes in adipose-derived mesenchymal stem cells cultured on fibrin scaffold</title><title>Journal of biosciences</title><addtitle>J Biosci</addtitle><addtitle>J Biosci</addtitle><description>In regenerative medicine, MSCs need to be pluripotent for better results. In this study, the effect of fibrin scaffold on expression of stemness genes was examined. Adipose-derived MSCs were cultured in tissue culture plates (2D) and 3-dimensional (3D) fibrin scaffolds. The effect of fibrin scaffold on proliferation of adipose-derived MSCs was evaluated by MTT assay. The expression of stemness genes (
OCT4
and
SOX2
) were evaluated by qRT-PCR, and flow cytometry was done for Nanog protein level. Cultured MSCs on fibrin scaffold were able to proliferate according to data obtained by MTT assay. Expression of
OCT4
and
SOX2
had a significant increase in cells were cultured in 3D condition compared to 2D condition (P < 0.05). Also, increased expression of Nanog protein in 3D culture was observed (P < 0.05).
OCT4
and
SOX2
in 3D condition increased two-fold and three-fold respectively in 2D and 3D conditions. Moreover, expression of Nanog increased 30% more than in 2D condition. Evaluation of important pluripotency regulators such as OCT4, SOX2, and Nanog showed that fibrin scaffolds are useful instruments to maintain stemness of MSCs, which is essential in field of stem cell therapy and regenerative medicine.</description><subject>Adipose Tissue - cytology</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>bone-marrow</subject><subject>Cell Adhesion</subject><subject>Cell Biology</subject><subject>Cell culture</subject><subject>Cell Culture Techniques</subject><subject>Cell Proliferation - physiology</subject><subject>Cell Survival - physiology</subject><subject>Cell therapy</subject><subject>Clinical Medicine</subject><subject>DNA</subject><subject>Evaluation</subject><subject>Fibrin</subject><subject>Fibrin scaffold</subject><subject>Flow cytometry</subject><subject>Gene expression</subject><subject>Gene Expression Regulation - physiology</subject><subject>Genes</subject><subject>Humans</subject><subject>Instruments</subject><subject>Klinisk medicin</subject><subject>Life Sciences</subject><subject>Life Sciences & Biomedicine - Other Topics</subject><subject>maintenance</subject><subject>Medical sciences</subject><subject>Mesenchymal stem cells</subject><subject>Mesenchymal Stem Cells - physiology</subject><subject>Microbiology</subject><subject>Microscopy, Electron, Scanning</subject><subject>Nanog</subject><subject>Nanog Homeobox Protein - genetics</subject><subject>Nanog Homeobox Protein - metabolism</subject><subject>Nanostructures</subject><subject>Nucleotide sequence</subject><subject>Oct-4 protein</subject><subject>OCT4</subject><subject>PCR</subject><subject>Plant Sciences</subject><subject>Pluripotency</subject><subject>Proliferation</subject><subject>Proteins</subject><subject>Regenerative medicine</subject><subject>Regulators</subject><subject>Scaffolds</subject><subject>SOX2</subject><subject>Stem cells</subject><subject>stemness</subject><subject>Surface Properties</subject><subject>Tissue culture</subject><subject>Tissue engineering</subject><subject>Tissue Scaffolds</subject><subject>Zoology</subject><issn>0250-5991</issn><issn>0973-7138</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNqFkU1PHDEMhqOqCCjwBzhUI_Wc4nwMmRwrRGklJC5wjvLhbGeZryYztPvvCeyyvZWTE_vxa1svIecMvjIAdZEZB9FQ4EABoAZafyDHoJWgionmY3nzl6TW7Ih8ynkNwLQUcEiOBK9rqeHymDxed_hkZwwV_p0S5tyOQzXGKs_YD-VbrbCEqh0qG9ppzEgDpvap8D1mHPyvTW-7V7ry2HW58ks3L6nUi05sXSqd2dsYxy6ckoNou4xnu3hCHr5f31_9oLd3Nz-vvt1SLyWbqQ615NZZG53AKOpauOC84yF6rxVYxqRtJCBTGFBGVEpq1ejolAugyoUnhG518x-cFmem1PY2bcxoW7NaJlNSq8VkNFzLRurCf9nyUxp_L5hnsx6XNJQVDZeNlhLU5TsU45wrJaBQfEv5NOacMO6nMzAvrpmta6a4Zl5dM3Vp-ryTXlyPYd_yZlMBxO6iUhpWmP7N_o_sM-dTpFg</recordid><startdate>20201201</startdate><enddate>20201201</enddate><creator>Mousavifard, Arezoo</creator><creator>Pourazizi Najafabadi, Elahe</creator><creator>Akhavan Rahnama, Mahshid</creator><creator>Anbarlou, Azadeh</creator><creator>Atashi, Amir</creator><general>Springer India</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7TM</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>F1W</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H99</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>L.F</scope><scope>L.G</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>M7P</scope><scope>MBDVC</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>ADTPV</scope><scope>AOWAS</scope><scope>F1U</scope></search><sort><creationdate>20201201</creationdate><title>Elevated expression of stemness genes in adipose-derived mesenchymal stem cells cultured on fibrin scaffold</title><author>Mousavifard, Arezoo ; Pourazizi Najafabadi, Elahe ; Akhavan Rahnama, Mahshid ; Anbarlou, Azadeh ; Atashi, Amir</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c441t-9d542abaafb3ef3553bdbcb2dfcc970a114a840e17ede4fe7749789fb7bd07943</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Adipose Tissue - cytology</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>bone-marrow</topic><topic>Cell Adhesion</topic><topic>Cell Biology</topic><topic>Cell culture</topic><topic>Cell Culture Techniques</topic><topic>Cell Proliferation - physiology</topic><topic>Cell Survival - physiology</topic><topic>Cell therapy</topic><topic>Clinical Medicine</topic><topic>DNA</topic><topic>Evaluation</topic><topic>Fibrin</topic><topic>Fibrin scaffold</topic><topic>Flow cytometry</topic><topic>Gene expression</topic><topic>Gene Expression Regulation - physiology</topic><topic>Genes</topic><topic>Humans</topic><topic>Instruments</topic><topic>Klinisk medicin</topic><topic>Life Sciences</topic><topic>Life Sciences & Biomedicine - Other Topics</topic><topic>maintenance</topic><topic>Medical sciences</topic><topic>Mesenchymal stem cells</topic><topic>Mesenchymal Stem Cells - physiology</topic><topic>Microbiology</topic><topic>Microscopy, Electron, Scanning</topic><topic>Nanog</topic><topic>Nanog Homeobox Protein - genetics</topic><topic>Nanog Homeobox Protein - metabolism</topic><topic>Nanostructures</topic><topic>Nucleotide sequence</topic><topic>Oct-4 protein</topic><topic>OCT4</topic><topic>PCR</topic><topic>Plant Sciences</topic><topic>Pluripotency</topic><topic>Proliferation</topic><topic>Proteins</topic><topic>Regenerative medicine</topic><topic>Regulators</topic><topic>Scaffolds</topic><topic>SOX2</topic><topic>Stem cells</topic><topic>stemness</topic><topic>Surface Properties</topic><topic>Tissue culture</topic><topic>Tissue engineering</topic><topic>Tissue Scaffolds</topic><topic>Zoology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mousavifard, Arezoo</creatorcontrib><creatorcontrib>Pourazizi Najafabadi, Elahe</creatorcontrib><creatorcontrib>Akhavan Rahnama, Mahshid</creatorcontrib><creatorcontrib>Anbarlou, Azadeh</creatorcontrib><creatorcontrib>Atashi, Amir</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Nucleic Acids Abstracts</collection><collection>ProQuest Health and Medical</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>ASFA: Marine Biotechnology Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>ProQuest Research Library</collection><collection>ProQuest Biological Science Journals</collection><collection>Research Library (Corporate)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>SwePub</collection><collection>SwePub Articles</collection><collection>SWEPUB Göteborgs universitet</collection><jtitle>Journal of biosciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mousavifard, Arezoo</au><au>Pourazizi Najafabadi, Elahe</au><au>Akhavan Rahnama, Mahshid</au><au>Anbarlou, Azadeh</au><au>Atashi, Amir</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Elevated expression of stemness genes in adipose-derived mesenchymal stem cells cultured on fibrin scaffold</atitle><jtitle>Journal of biosciences</jtitle><stitle>J Biosci</stitle><addtitle>J Biosci</addtitle><date>2020-12-01</date><risdate>2020</risdate><volume>45</volume><issue>1</issue><artnum>81</artnum><issn>0250-5991</issn><eissn>0973-7138</eissn><abstract>In regenerative medicine, MSCs need to be pluripotent for better results. In this study, the effect of fibrin scaffold on expression of stemness genes was examined. Adipose-derived MSCs were cultured in tissue culture plates (2D) and 3-dimensional (3D) fibrin scaffolds. The effect of fibrin scaffold on proliferation of adipose-derived MSCs was evaluated by MTT assay. The expression of stemness genes (
OCT4
and
SOX2
) were evaluated by qRT-PCR, and flow cytometry was done for Nanog protein level. Cultured MSCs on fibrin scaffold were able to proliferate according to data obtained by MTT assay. Expression of
OCT4
and
SOX2
had a significant increase in cells were cultured in 3D condition compared to 2D condition (P < 0.05). Also, increased expression of Nanog protein in 3D culture was observed (P < 0.05).
OCT4
and
SOX2
in 3D condition increased two-fold and three-fold respectively in 2D and 3D conditions. Moreover, expression of Nanog increased 30% more than in 2D condition. Evaluation of important pluripotency regulators such as OCT4, SOX2, and Nanog showed that fibrin scaffolds are useful instruments to maintain stemness of MSCs, which is essential in field of stem cell therapy and regenerative medicine.</abstract><cop>New Delhi</cop><pub>Springer India</pub><pmid>32554906</pmid><doi>10.1007/s12038-020-00050-5</doi></addata></record> |
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| ispartof | Journal of biosciences, 2020-12, Vol.45 (1), Article 81 |
| issn | 0250-5991 0973-7138 |
| language | eng |
| recordid | cdi_swepub_primary_oai_gup_ub_gu_se_294849 |
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| subjects | Adipose Tissue - cytology Biomedical and Life Sciences Biomedicine bone-marrow Cell Adhesion Cell Biology Cell culture Cell Culture Techniques Cell Proliferation - physiology Cell Survival - physiology Cell therapy Clinical Medicine DNA Evaluation Fibrin Fibrin scaffold Flow cytometry Gene expression Gene Expression Regulation - physiology Genes Humans Instruments Klinisk medicin Life Sciences Life Sciences & Biomedicine - Other Topics maintenance Medical sciences Mesenchymal stem cells Mesenchymal Stem Cells - physiology Microbiology Microscopy, Electron, Scanning Nanog Nanog Homeobox Protein - genetics Nanog Homeobox Protein - metabolism Nanostructures Nucleotide sequence Oct-4 protein OCT4 PCR Plant Sciences Pluripotency Proliferation Proteins Regenerative medicine Regulators Scaffolds SOX2 Stem cells stemness Surface Properties Tissue culture Tissue engineering Tissue Scaffolds Zoology |
| title | Elevated expression of stemness genes in adipose-derived mesenchymal stem cells cultured on fibrin scaffold |
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