Identification of Proteins Associated with the Early Restoration of Insulin Sensitivity After Biliopancreatic Diversion

• Published in: The journal of clinical endocrinology and metabolism 2020-11, Vol.105 (11), p.1-e4168
• Main Authors: Karlsson, Cecilia, Wallenius, Kristina, Walentinsson, Anna, Greasley, Peter J, Miliotis, Tasso, Hammar, Mårten, Iaconelli, Amerigo, Tapani, Sofia, Raffaelli, Marco, Mingrone, Geltrude, Carlsson, Björn
• Format: Article
• Language: English
• Subjects: Adult; afamin; Analysis; Apolipoprotein A; Apolipoprotein A-II; apolipoprotein A-IV; Apolipoproteins; bariatric surgery; biliopancreatic; Biliopancreatic Diversion; Blood Glucose - metabolism; Blood proteins; Body Mass Index; Body weight; Cardiovascular diseases; chronic kidney-disease; Clinical s; Diabetes mellitus (non-insulin dependent); diversion; Endocrinology & Metabolism; Endocrinology and Diabetes; Endokrinologi och diabetes; Ethylenediaminetetraacetic acid; Gastrointestinal surgery; Glucose Tolerance Test; Humans; Inflammation; Insulin; Insulin resistance; Insulin Resistance - physiology; Kidney diseases; Male; Mass spectrometry; Mass spectroscopy; Metabolism; Middle Aged; mild; Obesity; Obesity, Morbid - blood; Obesity, Morbid - surgery; Plasma; Plasma levels; Plasma proteins; polymorphism; Proteins; Proteomes; proteomic analysis; Proteomics; replication; resistance; Risk factors; Surgery; Type 2 diabetes
• ISSN: 0021-972X; 1945-7197
• DOI: 10.1210/clinem/dgaa558

Abstract Context Insulin resistance (IR) is a risk factor for type 2 diabetes, diabetic kidney disease, cardiovascular disease and nonalcoholic steatohepatitis. Biliopancreatic diversion (BPD) is the most effective form of bariatric surgery for improving insulin sensitivity. Objective To identify plasma proteins correlating with the early restoration of insulin sensitivity after BPD. Design Prospective single-center study including 20 insulin-resistant men with morbid obesity scheduled for BPD. Patient characteristics and blood samples were repeatedly collected from baseline up to 4 weeks postsurgery. IR was assessed by homeostatic model assessment for insulin resistance (HOMA-IR), Matsuda Index, and by studying metabolic profiles during meal tolerance tests. Unbiased proteomic analysis was performed to identify plasma proteins altered by BPD. Detailed plasma profiles were made on a selected set of proteins by targeted multiple reaction monitoring mass spectrometry (MRM/MS). Changes in plasma proteome were evaluated in relation to metabolic and inflammatory changes. Results BPD resulted in improved insulin sensitivity and reduced body weight. Proteomic analysis identified 29 proteins that changed following BPD. Changes in plasma levels of afamin, apolipoprotein A-IV (ApoA4), and apolipoprotein A-II (ApoA2) correlated significantly with changes in IR. Conclusion Circulating levels of afamin, ApoA4, and ApoA2 were associated with and may contribute to the rapid improvement in insulin sensitivity after BPD.