Quantitative proteomic analysis to identify differentially expressed proteins in patients with epilepsy

•Blood-based-biomarkers analysis in patients with new-onset seizure.•During follow-up, 63.6 % patients developed epilepsy, whereas 36.4 % had only single seizure.•A total of 1075 proteins were identified using TMT-based quantitative proteomic study in plasma samples.•The expression levels of 41 prot...

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Bibliographic Details
Published in:Epilepsy research 2021-08, Vol.174, p.106674-106674, Article 106674
Main Authors: Banote, Rakesh Kumar, Larsson, David, Berger, Evelin, Kumlien, Eva, Zelano, Johan
Format: Article
Language:English
Subjects:
Adult
Biomarkers
Blood biomarkers
Blood Proteins
Epilepsy - metabolism
epileptogenesis
Focal epilepsy
hippocampus
Humans
induction
long pentraxin ptx3
Mass spectrometry
Neurosciences
Neurosciences & Neurology
Neurovetenskaper
Pilot Projects
profiling
Proteomics - methods
Quantitative proteomic
Quantitative proteomic profiling
regulator
seizures
sonic hedgehog
subunit
systems-level analysis
Tandem mass tags (TMT)
tyrosine phosphorylation
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Summary:•Blood-based-biomarkers analysis in patients with new-onset seizure.•During follow-up, 63.6 % patients developed epilepsy, whereas 36.4 % had only single seizure.•A total of 1075 proteins were identified using TMT-based quantitative proteomic study in plasma samples.•The expression levels of 41 proteins were significantly altered in epilepsy patients.•The identified proteins might provide important information on the pathways associated with epileptogenesis. There is a great need for biomarkers in epilepsy, particularly markers of epileptogenesis. A first seizure will lead to epilepsy in 20–45 % of cases, but biomarkers that can identify these individuals are missing. The purpose of this study was to identify potential biomarkers of epilepsy/epileptogenesis in a cohort of adults with new-onset seizures, using quantitative proteomic analysis. Plasma was collected from 55 adults with new-onset seizures and sufficient follow-up to identify epilepsy. After a follow up period of two years, 63.6 % of the cohort had a diagnosis of epilepsy, whereas 36.4 % of patients only had a single seizure. Plasma proteins were extracted and labelled with tandem mass tags, then analyzed using mass spectrometry approach. Proteins that were up- or downregulated by ≥20 % and with a p-value of
ISSN:0920-1211
1872-6844
1872-6844
DOI:10.1016/j.eplepsyres.2021.106674