Quantification of Viral RNA in Multiple Pieces of Explant Liver Tissue Shows Distinct Focal Differences in Hepatitis B Infection

Abstract Hepatitis B virus (HBV) DNA and RNA were quantified by digital PCR assays in 20–30 tissue pieces from each of 4 liver explants with cirrhosis caused by HBV. The within-patient variability of HBV RNA levels between pieces was up to a 1000-fold. Core RNA and S RNA levels were similar and corr...

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Bibliographic Details
Published in:The Journal of infectious diseases 2022-09, Vol.226 (6), p.1036-1040
Main Authors: Rydell, Gustaf E, Prakash, Kasthuri, Larsson, Simon B, Skoglund, Catarina, Ringlander, Johan, Andersson, Maria E, Castedal, Maria, Norder, Heléne, Lindh, Magnus
Format: Article
Language:English
Subjects:
Brief Report
Circular DNA
Cirrhosis
Clinical Medicine
DNA viruses
Explants
Hepatitis
Hepatitis B
Hepatitis B surface antigen
Klinisk medicin
Liver
Liver cancer
Liver cirrhosis
Microbiology in the medical area
Mikrobiologi inom det medicinska området
Replication
Ribonucleic acid
RNA
RNA viruses
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Summary:Abstract Hepatitis B virus (HBV) DNA and RNA were quantified by digital PCR assays in 20–30 tissue pieces from each of 4 liver explants with cirrhosis caused by HBV. The within-patient variability of HBV RNA levels between pieces was up to a 1000-fold. Core RNA and S RNA levels were similar and correlated strongly when replication was high, supporting that transcription was from covalently closed circular DNA (cccDNA). By contrast, enhanced expression of S RNA relative to cccDNA and core RNA in patients with medium-high or low replication supports that HBV surface antigen (HBsAg) can be expressed mainly from integrated HBV DNA in such patients.
ISSN:0022-1899
1537-6613
1537-6613
DOI:10.1093/infdis/jiab469