Chemical imaging of aggressive basal cell carcinoma using time-of-flight secondary ion mass spectrometry

A set of basal cell carcinoma samples, removed by Mohs micrographic surgery and pathologically identified as having an aggressive subtype, have been analyzed using time-of-flight secondary ion mass spectrometry (SIMS). The SIMS analysis employed a gas cluster ion beam (GCIB) to increase the sensitiv...

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Bibliographic Details
Published in:Biointerphases 2018-06, Vol.13 (3), p.03B402-03B402
Main Authors: Munem, Marwa, Zaar, Oscar, Dimovska Nilsson, Kelly, Neittaanmäki, Noora, Paoli, John, Fletcher, John S
Format: Article
Language:English
Subjects:
analysis
Analytical Chemistry
Analytisk kemi
Basal Cell
Basic Medicine
Biopsy
Carcinoma
Carcinoma, Basal Cell - pathology
Dermatologi och venereologi
Dermatology and Venereal Diseases
Humans
Mass
Medicinska grundvetenskaper
methods
Microsurgery
pathology
Phospholipids
Phospholipids - analysis
Secondary Ion
Skin
Skin - pathology
Spectrometry
Spectrometry, Mass, Secondary Ion - methods
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Summary:A set of basal cell carcinoma samples, removed by Mohs micrographic surgery and pathologically identified as having an aggressive subtype, have been analyzed using time-of-flight secondary ion mass spectrometry (SIMS). The SIMS analysis employed a gas cluster ion beam (GCIB) to increase the sensitivity of the technique for the detection of intact lipid species. The GCIB also allowed these intact molecular signals to be maintained while surface contamination and delocalized chemicals were removed from the upper tissue surface. Distinct mass spectral signals were detected from different regions of the tissue (epidermis, dermis, hair follicles, sebaceous glands, scar tissue, and cancerous tissue) allowing mass spectral pathology to be performed. The cancerous regions of the tissue showed a particular increase in sphingomyelin signals that were detected in both positive and negative ion mode along with increased specific phosphatidylserine and phosphatidylinositol signals observed in negative ion mode. Samples containing mixed more and less aggressive tumor regions showed increased phosphatidylcholine lipid content in the less aggressive areas similar to a punch biopsy sample of a nonaggressive nodular lesion.
ISSN:1934-8630
1559-4106
1559-4106
DOI:10.1116/1.5016254