Pharmacologically active chemical composite of Musa balbisiana ameliorates oxidative stress, mitochondrial cellular respiration, and thereby metabolic dysfunction

• Published in: Journal of food biochemistry 2022-09, Vol.46 (9), p.e14347-n/a
• Main Authors: Sarma, Partha Pratim, Gurumayum, Nonibala, Samanta, Suman Kumar, Khound, Puspanjali, Kumari, Sima, Devi, Diptimayee, Barman, Jayanta, Banerjee, Sanjay Kumar, Devi, Rajlakshmi
• Format: Article
• Language: English
• Subjects: bioactive compounds; Biochemistry and Molecular Biology; Biokemi och molekylärbiologi; metabolic disorders; Musa balbisiana; nutraceutical; oxidative stress
• ISSN: 0145-8884; 1745-4514; 1745-4514
• DOI: 10.1111/jfbc.14347

The ripe fruit pulp of different Musa species is known for its excellent source of nutrient contents. Musa balbisiana (MB) is one such variety of Musa species, mainly found in the southern part of Asia, especially in the North‐eastern part of India, remains unexplored despite its continuous use by the local traditional healers. The present study focuses on identifying and quantifying the active chemicals present in the ripe fruit pulp of Musa balbisiana (RFPMB) to understand its combined efficacy and nutritional benefit to control human metabolic complications specially related to diabetes and cardiovascular disorder. Characterization and confirmation through targeted LC‐MS and HPLC‐PDA based assays followed by quantitative analysis led us to identify the major bioactive compounds in RFPMB as shikimic acid, p‐hydroxybenzoic acid, vanillic acid, ferulic acid, sinapic acid, caffeic acid, syringic acid, chlorogenic acid, trans‐cinnamic acid, and two essential fatty acids; linolenic acid and linoleic acid. The ripe fruit pulp is further analyzed to understand the nutritional and mineral content and found a substantial presence of calcium and potassium (15.74 ± 0.43 and 395.20 ± 9.5 mg/100 g of raw pulp, respectively) compared to other reported varieties. The active portion of RFPMB reduces the production of ROS, the expression of inflammatory marker genes TNF‐α and TGF‐β, and accelerates the mitochondrial oxygen consumption rate (OCR) by enhancing the basal respiration, maximal respiration, and ATP production capacity of the targeted cells. The present study concluded that, a particular phytopharmaceutical composition of RFPMB with 11‐biomarker compounds might be an efficacious formulation for developing a value‐added nutraceutical product in managing metabolic complications and its related oxidative stress. Practical applications This study has provided the prior information regarding the potential nutraceutical and phytochemical advantages of Musa balbisiana (MB) fruit pulp over other reported banana varieties. The HPLC‐based quantification will give a clear understanding of the food values in comparison of bioactive compounds present in the active fraction of RFPMB, which can be an effective phytopharmaceutical in combating metabolic disorders and oxidative stress. Overall this study will help to commercialize a value‐added product from this variety of banana with proper scientific validation. The present study focuses on the identification and quantificati