Early alterations in the postprandial VLDL1 apoB‐100 and apoB‐48 metabolism in men with strong heredity for type 2 diabetes

• Published in: Journal of internal medicine 2004-02, Vol.255 (2), p.273-279
• Main Authors: Johanson, E. H., Jansson, P.‐A., Gustafson, B., Lönn, L., Smith, U., Taskinen, M.‐R., Axelsen, M.
• Format: Article
• Language: English
• Subjects: Adult; Anthropometry; Apolipoprotein B-100; Apolipoprotein B-48; Apolipoproteins B - blood; apoproteins; Biological and medical sciences; Blood Glucose - metabolism; Cholesterol; Cholesterol, VLDL - blood; Cross-Sectional Studies; Diabetes Mellitus; Diabetes Mellitus, Type 2 - blood; Diabetes Mellitus, Type 2 - genetics; Diabetes. Impaired glucose tolerance; Endocrine pancreas. Apud cells (diseases); Endocrinopathies; Etiopathogenesis. Screening. Investigations. Target tissue resistance; General aspects; Genetic Predisposition to Disease; Glucose Tolerance Test; Humans; Insulin - blood; Insulin Resistance; Life Style; Male; MEDICAL AND HEALTH SCIENCES; Medical sciences; MEDICIN OCH HÄLSOVETENSKAP; Middle Aged; Postprandial Period - physiology; post‐prandial; Triglycerides - blood; triglyceride‐rich lipoproteins; Type 2/blood/genetics; VLDL/blood
• ISSN: 0954-6820; 1365-2796
• DOI: 10.1046/j.1365-2796.2003.01281.x

. Objectives.  To study the postprandial triglyceride‐rich lipoprotein (TRL) metabolism, specifically the concentrations of very low‐density lipoproteins (VLDL); from intestine (apoB‐48) and liver (apoB‐100), in men with normal fasting triglycerides but at increased risk of developing type 2 diabetes. Design.  Cross‐sectional study. Subjects and settings.  Sixteen healthy men with at least two first‐degree relatives with type 2 diabetes were individually matched with 16 control subjects without known diabetes heredity for: age, body mass index, and fasting triglyceride level. They underwent an 8‐h meal tolerance test (919 kcal, 51 g fat) during which lipoproteins were separated by density gradient ultracentrifugation. They were characterized by euglycaemic hyperinsulinaemic clamp, peak VO2, 7‐day diet registration and computed tomography. Results.  The relatives were, as expected, more insulin resistant than the controls and had increased concentration of postprandial VLDL1 particles (49% higher for VLDL1 apoB‐48, P = 0.04 and 21% higher for VLDL1 apoB‐100, P = 0.048). The elevation was related to insulin sensitivity, but not to lifestyle and body composition. Moreover, the concentration of postprandial triglycerides in VLDL1 fraction was inversely related to low‐density lipoprotein (LDL) size in both relatives (rs = −0.60, P = 0.03) and controls (rs = −0.72, P = 0.004). There were no differences in the concentration of triglycerides or apoB‐48 and apoB‐100 particles in the other fractions (plasma, chylomicron or VLDL2). Conclusion.  Increased postprandial concentration of TRLs in the VLDL1 fraction seems to be present at an early stage in the development of diabetes and probably contributes to the excess risk of future coronary events in insulin‐resistant men.