Complement activation in prestorage leucocyte-filtered plasma

Complement activation and generation of pro‐inflammatory cytokines occur during storage of blood components. Prestorage leucocyte filtration of platelet concentrates and red cells diminishes the accumulation of leucocyte‐derived cytokines during storage, however, transfusion reactions are not elimin...

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Bibliographic Details
Published in:Transfusion medicine (Oxford, England) England), 2004-02, Vol.14 (1), p.45-52
Main Authors: Hyllner, M., Tylman, M., Bengtson, J. P., Rydberg, L., Bengtsson, A.
Format: Article
Language:English
Subjects:
analysis
Blood
Blood - immunology
Blood Preservation
blood storage
Blood Transfusion
Blood Transfusion - standards
Cell Separation
Complement Activation
Complement C3a
Complement C3a - metabolism
Complement Membrane Attack Complex
Complement System Proteins
Complement System Proteins - metabolism
cytokine generation
Cytokines
Cytokines - analysis
Filtration
Glycoproteins
Glycoproteins - metabolism
Humans
immunology
Interleukin-8
Interleukin-8 - metabolism
Leukocytes
MEDICAL AND HEALTH SCIENCES
MEDICIN OCH HÄLSOVETENSKAP
metabolism
Plasma
Plasma - immunology
universal leucodepletion
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Summary:Complement activation and generation of pro‐inflammatory cytokines occur during storage of blood components. Prestorage leucocyte filtration of platelet concentrates and red cells diminishes the accumulation of leucocyte‐derived cytokines during storage, however, transfusion reactions are not eliminated. We investigated inflammatory mediator release during storage of plasma and whole blood and the effect of prestorage leucocyte filtration of plasma. Twenty‐four blood units were collected from healthy blood donors and stored for 35 days. Eight units were stored as whole blood, eight units as plasma and eight units as prestorage filtered plasma. Samples were collected weekly for analyses of potassium, leucocytes, free plasma haemoglobin, complement activation (C3a and SC5b‐9) and pro‐inflammatory cytokines [interleukin (IL)‐6, IL‐8 and tumor necrosis factor (TNF)‐α]. Elevated levels of C3a and SC5b‐9 were registered in filtered plasma, from the beginning of storage. C3a levels increased during storage. There was a higher rate of change during storage in C3a (P 
ISSN:0958-7578
1365-3148
DOI:10.1111/j.0958-7578.2004.00479.x