Relationships between Distinct Blood Monocyte Subsets and Migrating Intestinal Lymph Dendritic Cells In Vivo under Steady-State Conditions

The origins of dendritic cells (DCs) are poorly understood. In inflammation, DCs can arise from blood monocytes (M(O)s), but their steady-state origin may differ, as shown for Langerhans cells. Two main subsets of M(O)s, defined by expression of different chemokine receptors, CCR2 and CX(3)CR1, have...

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Bibliographic Details
Published in:Journal of Immunology 2006-04, Vol.176 (7), p.4155-4162
Main Authors: Yrlid, Ulf, Jenkins, Christopher D, MacPherson, G. Gordon
Format: Article
Language:English
Subjects:
Animals
Antigens
Antigens, CD - metabolism
Cell Differentiation
Cell Division
Cell Movement
cytology
Dendritic Cells
Dendritic Cells - cytology
Intestines
Intestines - cytology
Lymph Nodes
Lymph Nodes - cytology
Male
metabolism
Microbiology in the medical area
Mikrobiologi inom det medicinska området
Monocytes
Monocytes - cytology
Monocytes - metabolism
Rats
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Summary:The origins of dendritic cells (DCs) are poorly understood. In inflammation, DCs can arise from blood monocytes (M(O)s), but their steady-state origin may differ, as shown for Langerhans cells. Two main subsets of M(O)s, defined by expression of different chemokine receptors, CCR2 and CX(3)CR1, have been described in mice and humans. Recent studies have identified the inflammatory function of CCR2(high)CX(3)CR1(low) M(O)s but have not defined unambiguously the origin and fate of CCR2(low)CX(3)CR1(high) cells. In this study, we show that rat M(O)s can also be divided into CCR2(high)CX(3)CR1(low)(CD43(low)) and CCR2(low)CX(3)CR1(high)(CD43(high)) subsets with distinct migratory properties in vivo. Using whole body perfusion to obtain M(O)s, including the marginating pool, we show by adoptive transfer that CD43(low) M(O)s can differentiate into CD43(high) M(O)s in blood without cell division. By adoptive transfer of blood M(O)s followed by collection of pseudoafferent lymph, we show for the first time that a small proportion of intestinal lymph DCs are derived from CCR2(low)CX(3)CR1(high)(CD43(high)) blood M(O)s in vivo under steady-state conditions. This study confirms one of the possible origins of CCR2(low)CX(3)CR1(high) blood M(O)s and indicate that they may contribute to migratory intestinal DCs in vivo in the absence of inflammatory stimuli.
ISSN:0022-1767
1550-6606
1365-2567
DOI:10.4049/jimmunol.176.7.4155