Expression Profile of Galectin-1 and Galectin-3 Molecules in Different Subtypes of Chronic Lymphocytic Leukemia

• Published in: Cancer investigation 2010-07, Vol.28 (7), p.717-725
• Main Authors: Asgarian-Omran, Hossein, Forghani, Parvin, Hojjat-Farsangi, Mohammad, Roohi, Azam, Sharifian, Ramazan Ali, Razavi, Seyed Mohsen, Jeddi-Tehrani, Mahmood, Rabbani, Hodjatallah, Shokri, Fazel
• Format: Article
• Language: English
• Subjects: Aged; Chronic lymphocytic leukemia; Disease Progression; Female; Flow Cytometry; Galectin; Galectin 1 - genetics; Galectin 1 - metabolism; Galectin 3 - genetics; Galectin 3 - metabolism; Gene Expression Profiling; Humans; Leukemia, Lymphocytic, Chronic, B-Cell - genetics; Male; Medicin och hälsovetenskap; Middle Aged; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger - metabolism; RT-PCR
• ISSN: 0735-7907; 1532-4192; 1532-4192
• DOI: 10.3109/07357907.2010.494319

ABSTRACT Galectin-1 (Gal-1) and galectin-3 (Gal-3) molecules are involved in many vital, biological, and pathological processes. In this study the expression pattern of these two molecules was investigated in leukemic cells from 85 Iranian chronic lymphocytic leukemia (CLL) patients, classified into indolent, progressive, mutated, and unmutated subtypes by semiquantitative reverse transcriptase polymerase chain reaction (RT-PCR) and flow cytometry methods. Our results showed significant downregulation of Gal-3, but not Gal-1, in CLL patients compared with normal subjects (p < .001). Higher representation of Gal-3 mRNA was observed in indolent patients compared with the progressive group (p < .05). Our findings imply a regulatory role for Gal-3 gene in initiation and/or progression of CLL.