Herpes simplex virus type 1 entry into epithelial MDCKII cells: role of VASP activities

VASP is an actin-regulatory protein that links signalling to remodelling of the cytoskeleton. We investigated the role of VASP during entry of herpes simplex viruses into epithelial MDCKII cells. As VASP functions are regulated by phosphorylations, the phosphorylation pattern was determined upon inf...

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Bibliographic Details
Published in:Journal of general virology 2010-09, Vol.91 (Pt 9), p.2152-2157
Main Authors: Jaeger, Verena, Hoppe, Sven, Petermann, Philipp, Liebig, Timo, Jansen, Matthias K, Renné, Thomas, Knebel-Mörsdorf, Dagmar
Format: Article
Language:English
Subjects:
Amino Acid Substitution
Animals
Cell Adhesion Molecules - antagonists & inhibitors
Cell Adhesion Molecules - chemistry
Cell Adhesion Molecules - genetics
Cell Adhesion Molecules - physiology
Cell Line
Dogs
Gene Deletion
Gene Expression
Herpes Simplex - etiology
Herpes Simplex - physiopathology
Herpes Simplex - virology
Herpes simplex virus 1
Herpesvirus 1, Human - pathogenicity
Herpesvirus 1, Human - physiology
Medicin och hälsovetenskap
Microfilament Proteins - antagonists & inhibitors
Microfilament Proteins - chemistry
Microfilament Proteins - genetics
Microfilament Proteins - physiology
Mutagenesis, Site-Directed
Mutation
Phosphoproteins - antagonists & inhibitors
Phosphoproteins - chemistry
Phosphoproteins - genetics
Phosphoproteins - physiology
Phosphorylation
Recombinant Proteins - chemistry
Recombinant Proteins - genetics
Recombinant Proteins - metabolism
RNA Interference
Serine - chemistry
Transfection
Virus Internalization
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Summary:VASP is an actin-regulatory protein that links signalling to remodelling of the cytoskeleton. We investigated the role of VASP during entry of herpes simplex viruses into epithelial MDCKII cells. As VASP functions are regulated by phosphorylations, the phosphorylation pattern was determined upon infection. Phosphorylated VASP decreased temporarily at 15 and 30 min after infection. The impact of phosphorylated VASP was addressed by overexpression of phosphomimetic VASP mutants. Our results revealed that phosphorylated VASP slightly reduced the number of infected cells. Expression studies with deletion mutants further indicated minor effects of VASP on infection efficiency, whereas RNA interference studies demonstrated that reduced VASP expression did not suppress infection. We conclude that VASP activities alone may contribute to herpes simplex virus infection to only a minor extent.
ISSN:0022-1317
1465-2099
1465-2099
DOI:10.1099/vir.0.021055-0