Platelet‐ and erythrocyte‐derived microparticles trigger thrombin generation via factor XIIa

See also Shapiro S, Laffan M. Making contact with microparticles. This issue, pp 1352–4. Summary.  Background:  The procoagulant properties of microparticles (MPs) are due to the of the presence of phosphatidylserine (PS) and tissue factor (TF) on their surface. The latter has been demonstrated espe...

Full description

Saved in:
Bibliographic Details
Published in:Journal of thrombosis and haemostasis 2012-07, Vol.10 (7), p.1355-1362
Main Authors: VAN DER MEIJDEN, P. E. J., VAN SCHILFGAARDE, M., VAN OERLE, R., RENNÉ, T., TEN CATE, H., SPRONK, H. M. H.
Format: Article
Language:English
Subjects:
Blood Platelets - physiology
Cell-Derived Microparticles
Erythrocytes
Erythrocytes - physiology
factor XII
Factor XIIa - physiology
Flow Cytometry
Humans
Immunophenotyping
Medicin och hälsovetenskap
microparticles
Reference Values
Thrombin - biosynthesis
thrombin generation
tissue factor
Transplants & implants
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:See also Shapiro S, Laffan M. Making contact with microparticles. This issue, pp 1352–4. Summary.  Background:  The procoagulant properties of microparticles (MPs) are due to the of the presence of phosphatidylserine (PS) and tissue factor (TF) on their surface. The latter has been demonstrated especially on MPs derived from monocytes. Objectives:  To investigate the relative contribution of TF and factor (F)XII in initiating coagulation on MPs derived from monocytes, platelets and erythrocytes. Methods:  Microparticles were isolated from calcium ionophore‐stimulated platelets, erythrocytes and monocytic THP‐1 cells. MPs were quantified, characterized for cell‐specific antigens and analyzed for TF, PS exposure and their thrombin‐generating potential. Results:  The MP number was not proportional to PS exposure and the majority of the MPs exposed PS. TF activity was undetectable on platelet‐ and erythrocyte‐derived MPs (
ISSN:1538-7933
1538-7836
1538-7836
DOI:10.1111/j.1538-7836.2012.04758.x