Matrix metalloproteinases in the restorative proctocolectomy pouch of pediatric ulcerative colitis

• Published in: World journal of gastroenterology : WJG 2012-08, Vol.18 (30), p.4028-4036
• Main Authors: Makitalo, L, Piekkala, M, Ashorn, M, Pakarinen, M, Koivusalo, A, Karikoski, R, Natunen, J, Saarialho-Kere, U, Rintala, R, Kolho, KL
• Format: Article
• Language: English
• Subjects: Adolescent; Adult; Brief; Child; Child, Preschool; Colitis, Ulcerative - enzymology; Colitis, Ulcerative - surgery; Colonic Pouches; Female; Humans; Intestinal Mucosa - enzymology; Male; Matrix Metalloproteinases - metabolism; Medicin och hälsovetenskap; MMPs; Pouchitis - enzymology; Proctocolectomy, Restorative; TIMPs; Tissue Inhibitor of Metalloproteinases - metabolism; Young Adult; 基质金属蛋白酶-3; 小儿; 恢复性; 溃疡性结肠炎; 直肠; 红细胞沉降率
• ISSN: 1007-9327; 2219-2840; 2219-2840
• DOI: 10.3748/wjg.v18.i30.4028

AIM：To investigate matrix metalloproteinases（MMPs） and their tissue inhibitors（TIMPs） in pouch mucosa of pediatric onset ulcerative colitis（UC）.METHODS：In this cross-sectional study,28 patients with pediatric onset UC underwent ileal pouch biopsy 13 years（median） after proctocolectomy.Expression of MMPs-3,-7,-8,-9,-12 and-26 and TIMPs-1,-2 and-3 in samples was examined using immunohistochemichal methods,and another biopsy was used to evaluate the grade of histological inflammation.Two investigators independently graded the immunohistochemical specimens in a semiquantitative fashion,using a scale marking staining intensity as follows：0 = less than 20 positive cells;1 = 20-50 positive cells;2 = 50-200 positive cells;3 = over 20 positive cells.Fecal calprotectin and blood inflammatory markers [serum C-reactive protein（CRP） and erythrocyte sedimentation rate] were determined during a follow-up visit to examine correlations between these markers and the expression of MMPs and TIMPs.RESULTS：Of the 28 patients with pediatric onset UC,nine had not experienced pouchitis,whereas thirteen reported a single episode,and six had recurrent pouchitis（≥ 4 episodes）.At the time of the study,six patients required metronidazole.In all of the others,the most recent episode of pouchitis had occurred over one month earlier,and none were on antibiotics.Only four samples depicted no sign of inflammation,and these were all from patients who had not had pouchitis.Two samples were too small to determine the grade of inflammation,but both had suffered pouchitis,the other recurrent.No sample depicted signs of colonic metaplasia.Most pouch samples showed expression of epithelial（e） and stromal（s） MMP-3（e,n = 22;s,n = 20）,MMP-7（e,n = 28;s,n = 27）,MMP-12（e,n = 20;s,n =24）,TIMP-2（e,n = 23;s,n = 23） and MMP-3（e,n = 23;s,n = 28） but MMP-8（e,n = 0;s,n = 1）,MMP-9（e,n = 0;s,n = 9） and MMP-26（e,n = 0;s,n = 3） and TIMP-1（n = 0,both） were lacking.In samples with low grade of inflammatory activity,the epithelial MMP-3 and MMP-7 expression was increased（r =-0.614 and r =-0.472,respectively,P 〈 0.05 in both）.MMPs and TIMPs did not correlate with the markers of inflammation,fecal calprotectin,erythrocyte sedimentation rate,or CRP,with the exception of patients with low fecal calprotectin（〈 100 μg/g） in whom a higher expression of epithelial MMP-7 was found no differences in MMPor TIMP-profiles were seen in patients with a history of pouchitis compared to ones with no such episodes.Anastomosis with eit