In vivo SPECT and ex vivo autoradiographic brain imaging of the novel selective CB1 receptor antagonist radioligand [125 I]SD7015 in CB1 knock-out and wildtype mouse

• Published in: Brain research bulletin 2013-02, Vol.91, p.46-51
• Main Authors: Máthé, Domokos, Horváth, Ildikó, Szigeti, Krisztián, Donohue, Sean R, Pike, Victor W, Jia, Zisheng, Ledent, Catherine, Palkovits, Miklós, Freund, Tamás F, Halldin, Christer, Gulyás, Balázs
• Format: Article
• Language: English
• Subjects: [125I]SD7015; Animals; Autoradiography; Brain - diagnostic imaging; Brain - metabolism; Endocannabinoid CB1 receptor (CB1R); Image Processing, Computer-Assisted; Knock-out CB1R-/- mouse; Medicin och hälsovetenskap; Mice; Mice, Inbred C57BL; Mice, Knockout; Molecular imaging biomarker; Multimodal Imaging - methods; Multiplexed multipinhole dedicated small animal SPECT/CT system; Neuroimaging - methods; Neurology; Positron-Emission Tomography; Pyrazoles - pharmacology; Radiopharmaceuticals - pharmacology; Receptor, Cannabinoid, CB1 - antagonists & inhibitors; Single photon emission computed tomography (SPECT); Tomography, X-Ray Computed
• ISSN: 0361-9230; 1873-2747; 1873-2747
• DOI: 10.1016/j.brainresbull.2013.01.001

Highlights ► CB1 receptors (CB1 Rs) are highly expressed neuroreceptors in the primate brain. ► [125 I]SD7015 is a novel high-affinity lipophilic CB1 R antagonist SPECT radioligand. ► We scanned the brain uptake of [125 I]SD7015 in wildtype and CB1 R knock-out mice. ► [125 I]SD7015 brain uptake in wildtype mice is significantly higher than in KO mice. ► [125 I]SD7015 appears to be a promising SPECT radioligand for visualising CB1 Rs.