In vitro assessment of platelet concentrates with multiple electrode aggregometry

• Published in: Platelets (Edinburgh) 2015-01, Vol.26 (2), p.132-137
• Main Authors: Shams Hakimi, Caroline, Hesse, Camilla, Wallén, Håkan, Boulund, Fredrik, Grahn, Ammi, Jeppsson, Anders
• Format: Article
• Language: English
• Subjects: Aggregometry; apheresis; Blood Preservation - methods; buffy coat; Clinical Medicine; Electrodes; Humans; In Vitro Techniques; Klinisk medicin; Medicin och hälsovetenskap; Platelet Aggregation; Platelet Function Tests - instrumentation; Platelet Function Tests - methods; Platelet Transfusion; Plateletpheresis; platelets; Time Factors
• ISSN: 0953-7104; 1369-1635; 1369-1635
• DOI: 10.3109/09537104.2014.898141

ABSTRACT Storage impairs platelet function. It was hypothesized that multiple electrode aggregometry in vitro could be used to follow aggregability in platelet concentrates over time and that the results predict the efficacy of platelet transfusion in an ex vivo transfusion model. In vitro platelet aggregability was assessed in apheresis and pooled buffy coat platelet concentrates (BCs) (n = 13 each) using multiple electrode aggregometry with different agonists 1, 3, 5 and 7 days after preparation. In the ex vivo transfusion model, whole blood samples from nine healthy volunteers were collected every second day. The samples were supplemented with stored platelets (+146 × 109 × l−1) from the same unit 1, 3, 5 and 7 days after preparation. Platelet aggregability was assessed in the concentrate and in the whole blood samples before and after platelet supplementation. There was a continuous reduction in in vitro platelet aggregability over time in both apheresis and pooled BCs. The same pattern was observed after ex vivo addition of apheresis and pooled BCs to whole blood samples. The best correlation between in vitro aggregability and changes in aggregation after addition was achieved with collagen as agonist (r = 0.67, p