S18 family of mitochondrial ribosomal proteins: evolutionary history and Gly132 polymorphism in colon carcinoma

S18 family of mitochondrial ribosomal proteins (MRPS18, S18) consists of three members, S18-1 to -3. Earlier, we found that overexpression of S18-2 protein resulted in immortalization and eventual transformation of primary rat fibroblasts. The S18-1 and -3 have not exhibited such abilities. To under...

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Bibliographic Details
Published in:Oncotarget 2016-08, Vol.7 (34), p.55649-55662
Main Authors: Mushtaq, Muhammad, Ali, Raja Hashim, Kashuba, Vladimir, Klein, George, Kashuba, Elena
Format: Article
Language:English
Subjects:
Colonic Neoplasms - genetics
Evolution, Molecular
Evolutionary trace analysis
Genetic polymorphism
Humans
Medicin och hälsovetenskap
Mitochondrial Proteins - genetics
Mitochondrial ribosomal proteins
MRPS18 family
Mutation
Phylogenetic analysis
Phylogeny
Polymorphism, Genetic
Research Paper
Ribosomal Proteins - genetics
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Summary:S18 family of mitochondrial ribosomal proteins (MRPS18, S18) consists of three members, S18-1 to -3. Earlier, we found that overexpression of S18-2 protein resulted in immortalization and eventual transformation of primary rat fibroblasts. The S18-1 and -3 have not exhibited such abilities. To understand the differences in protein properties, the evolutionary history of S18 family was analyzed. The S18-3, followed by S18-1 and S18-2 emerged as a result of ancient gene duplication in the root of eukaryotic species tree, followed by two metazoan-specific gene duplications. However, the most conserved metazoan S18 homolog is the S18-1; it shares the most sequence similarity with S18 proteins of bacteria and of other eukaryotic clades. Evolutionarily conserved residues of S18 proteins were analyzed in various cancers. S18-2 is mutated at a higher rate, compared with S18-1 and -3 proteins. Moreover, the evolutionarily conserved residue, Gly132 of S18-2, shows genetic polymorphism in colon adenocarcinomas that was confirmed by direct DNA sequencing.Concluding, S18 family represents the yet unexplored important mitochondrial ribosomal proteins.
ISSN:1949-2553
1949-2553
DOI:10.18632/oncotarget.10957