ICAM-1 Binding Rhinoviruses Enter HeLa Cells via Multiple Pathways and Travel to Distinct Intracellular Compartments for Uncoating

Of the more than 150 human rhinovirus (RV) serotypes, 89 utilize intercellular adhesion molecule-1 (ICAM-1) for cell entry. These belong either to species A or B. We recently demonstrated that RV-B14 and RV-A89, despite binding this same receptor, are routed into distinct endosomal compartments for...

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Bibliographic Details
Published in:Viruses 2017-04, Vol.9 (4), p.68
Main Authors: Ganjian, Haleh, Zietz, Christin, Mechtcheriakova, Diana, Blaas, Dieter, Fuchs, Renate
Format: Article
Language:English
Subjects:
Actin
Antibodies
Binding sites
Biological Transport
Cell adhesion
Cholesterol
Clathrin
Cold
Cytosol
Drug delivery
Drugs
Dynamin
Dynein
HeLa Cells
Humans
Hydrogen-Ion Concentration
Infections
Intercellular adhesion molecule 1
Intercellular Adhesion Molecule-1 - metabolism
Intracellular
Medicin och hälsovetenskap
pH effects
Receptors, Virus - metabolism
Rhinovirus
Rhinovirus - physiology
Ribonucleic acid
RNA
Serotypes
Temperature
Temperature effects
Travel
Uncoating
Virus Attachment
Virus Internalization
Virus Uncoating
Viruses
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Summary:Of the more than 150 human rhinovirus (RV) serotypes, 89 utilize intercellular adhesion molecule-1 (ICAM-1) for cell entry. These belong either to species A or B. We recently demonstrated that RV-B14 and RV-A89, despite binding this same receptor, are routed into distinct endosomal compartments for release of their RNA into the cytosol. To gain insight into the underlying mechanism we now comparatively investigate the port of entry, temperature-dependence of uncoating, and intracellular routing of RV-B3, RV-B14, RV-A16, and RV-A89 in HeLa cells. The effect of various drugs blocking distinct stages on the individual pathways was determined via comparing the number of infected cells in a TissueFaxs instrument. We found that RV-B14 and RV-A89 enter via clathrin-, dynamin-, and cholesterol-dependent pathways, as well as by macropinocytosis. Drugs interfering with actin function similarly blocked entry of all four viruses, indicating their dependence on a dynamic actin network. However, uniquely, RV-A89 was able to produce progeny when internalized at 20 °C followed by neutralizing the endosomal pH and further incubation at 37 °C. Blocking dynein-dependent endosomal transport prevented uncoating of RV-A16 and RV-A89, but not of RV-B3 and RV-B14, indicative for routing of RV-A16 and RV-A89 into the endocytic recycling compartment for uncoating. Our results call for caution when developing drugs aimed at targeting entry or intracellular trafficking of all rhinovirus serotypes.
ISSN:1999-4915
1999-4915
DOI:10.3390/v9040068