The structure of the exopolysaccharide produced by the halophilic Archaeon Haloferax mediterranei strain R4 (ATCC 33500)

The halophilic Archaeon Haloferax mediterranei exudes into the growth medium a high molecular weight sulfated polysaccharide. The structure of the repeating unit of this polymer was determined by a combination of glycose, methylation, and sulfate analysis, periodate oxidation, and 1D and 2D NMR spec...

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Bibliographic Details
Published in:Carbohydrate research 1996-12, Vol.295 (1-4), p.147-156
Main Authors: Parolis, H, Parolis, L A, Boán, I F, Rodríguez-Valera, F, Widmalm, G, Manca, M C, Jansson, P E, Sutherland, I W
Format: Article
Language:English
Subjects:
Acetylglucosamine - analogs & derivatives
Acetylglucosamine - analysis
Archaea - chemistry
Carbohydrate Conformation
Carbohydrate Sequence
Glucose - analysis
Magnetic Resonance Spectroscopy
Mannose - analysis
Medicin och hälsovetenskap
Molecular Sequence Data
Oligosaccharides - chemistry
Periodic Acid - metabolism
Polysaccharides, Bacterial - chemistry
Repetitive Sequences, Nucleic Acid
Sequence Analysis
Spectrometry, Mass, Fast Atom Bombardment
Sulfuric Acid Esters - chemistry
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Summary:The halophilic Archaeon Haloferax mediterranei exudes into the growth medium a high molecular weight sulfated polysaccharide. The structure of the repeating unit of this polymer was determined by a combination of glycose, methylation, and sulfate analysis, periodate oxidation, and 1D and 2D NMR spectroscopic analysis of the native and periodate-oxidised/reduced polysaccharides. The location of the sulfate group was established from the 1H and 13C NMR data. The structure of the repeating unit of the polysaccharide may be written as [formula: see text]
ISSN:0008-6215
DOI:10.1016/S0008-6215(96)00219-4