Transcriptomic changes with increasing algal symbiont reveal the detailed process underlying establishment of coral-algal symbiosis

• Published in: Scientific reports 2018-11, Vol.8 (1), p.16802-11, Article 16802
• Main Authors: Yuyama, Ikuko, Ishikawa, Masakazu, Nozawa, Masafumi, Yoshida, Masa-aki, Ikeo, Kazuho
• Format: Article
• Language: English
• Subjects: 38/39; 38/77; 631/208/199; 631/337/2019; 631/601/18; Algae; Corals; Digestive enzymes; Endocytosis; Gene expression; Humanities and Social Sciences; Immune system; Inoculation; Medicin och hälsovetenskap; multidisciplinary; Ribonucleic acid; RNA; Science; Science (multidisciplinary); Symbiodinium; Symbionts; Symbiosis
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-018-34575-5

To clarify the establishment process of coral-algal symbiotic relationships, coral transcriptome changes during increasing algal symbiont densities were examined in juvenile corals following inoculation with the algae Symbiodinium goreaui (clade C) and S. trenchii (clade D), and comparison of their transcriptomes with aposymbiotic corals by RNA-sequencing. Since Symbiodinium clades C and D showed very different rates of density increase, comparisons were made of early onsets of both symbionts, revealing that the host behaved differently for each. RNA-sequencing showed that the number of differentially-expressed genes in corals colonized by clade D increased ca. two-fold from 10 to 20 days, whereas corals with clade C showed unremarkable changes consistent with a slow rate of density increase. The data revealed dynamic metabolic changes in symbiotic corals. In addition, the endocytosis pathway was also upregulated, while lysosomal digestive enzymes and the immune system tended to be downregulated as the density of clade D algae increased. The present dataset provides an enormous number of candidate symbiosis-related molecules that exhibit the detailed process by which coral-algal endosymbiosis is established.