Rapid diagnostic assay for detection of cellulose in urine as biomarker for biofilm-related urinary tract infections

The ability of uropathogenic Escherichia coli (UPEC) to adopt a biofilm lifestyle in the urinary tract is suggested as one cause of recurrent urinary tract infections (UTIs). A clinical role of UPEC biofilm is further supported by the presence of bacterial aggregates in urine of UTI patients. Yet, n...

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Bibliographic Details
Published in:NPJ biofilms and microbiomes 2018-10, Vol.4 (1), p.26-7, Article 26
Main Authors: Antypas, Haris, Choong, Ferdinand X., Libberton, Ben, Brauner, Annelie, Richter-Dahlfors, Agneta
Format: Article
Language:English
Subjects:
631/1647
631/326/2521
631/326/46
Biofilms
Biomarkers
Biomedical and Life Sciences
Cellulose
Conformation
Extracellular matrix
Life Sciences
Medical Microbiology
Medicin och hälsovetenskap
Microbial Ecology
Microbial Genetics and Genomics
Microbiology
Multivariate analysis
Patients
Urinary tract
Urinary tract diseases
Urinary tract infections
Urine
Urogenital system
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Summary:The ability of uropathogenic Escherichia coli (UPEC) to adopt a biofilm lifestyle in the urinary tract is suggested as one cause of recurrent urinary tract infections (UTIs). A clinical role of UPEC biofilm is further supported by the presence of bacterial aggregates in urine of UTI patients. Yet, no diagnostics exist to differentiate between the planktonic and biofilm lifestyle of bacteria. Here, we developed a rapid diagnostic assay for biofilm-related UTI, based on the detection of cellulose in urine. Cellulose, a component of biofilm extracellular matrix, is detected by a luminescent-conjugated oligothiophene, which emits a conformation-dependent fluorescence spectrum when bound to a target molecule. We first defined the cellulose-specific spectral signature in the extracellular matrix of UPEC biofilm colonies, and used these settings to detect cellulose in urine. To translate this optotracing assay for clinical use, we composed a workflow that enabled rapid isolation of urine sediment and screening for the presence of UPEC-derived cellulose in
ISSN:2055-5008
2055-5008
DOI:10.1038/s41522-018-0069-y