Two Aldehyde Clearance Systems Are Essential to Prevent Lethal Formaldehyde Accumulation in Mice and Humans

• Published in: Molecular cell 2020-12, Vol.80 (6), p.996-1012.e9
• Main Authors: Dingler, Felix A., Wang, Meng, Mu, Anfeng, Millington, Christopher L., Oberbeck, Nina, Watcham, Sam, Pontel, Lucas B., Kamimae-Lanning, Ashley N., Langevin, Frederic, Nadler, Camille, Cordell, Rebecca L., Monks, Paul S., Yu, Rui, Wilson, Nicola K., Hira, Asuka, Yoshida, Kenichi, Mori, Minako, Okamoto, Yusuke, Okuno, Yusuke, Muramatsu, Hideki, Shiraishi, Yuichi, Kobayashi, Masayuki, Moriguchi, Toshinori, Osumi, Tomoo, Kato, Motohiro, Miyano, Satoru, Ito, Etsuro, Kojima, Seiji, Yabe, Hiromasa, Yabe, Miharu, Matsuo, Keitaro, Ogawa, Seishi, Göttgens, Berthold, Hodskinson, Michael R.G., Takata, Minoru, Patel, Ketan J.
• Format: Article
• Language: English
• Subjects: Adolescent; ageing; Alcohol Dehydrogenase - genetics; Aldehyde Dehydrogenase, Mitochondrial - genetics; Aldehydes - blood; Animals; bone marrow failure; cancer; Child; Child, Preschool; DNA Adducts - genetics; DNA damage; DNA Damage - drug effects; DNA Repair - drug effects; Female; formaldehyde; Formaldehyde - blood; Formaldehyde - toxicity; hematopoiesis; Hematopoiesis - genetics; hematopoietic stem cells; Hematopoietic Stem Cells - metabolism; Humans; immunodeficiency; Infant; Leukemia - blood; Leukemia - genetics; Leukemia - pathology; Male; Medicin och hälsovetenskap; Mice; mutagenesis; Mutation - genetics; oncometabolite; Substrate Specificity
• ISSN: 1097-2765; 1097-4164; 1097-4164
• DOI: 10.1016/j.molcel.2020.10.012

Reactive aldehydes arise as by-products of metabolism and are normally cleared by multiple families of enzymes. We find that mice lacking two aldehyde detoxifying enzymes, mitochondrial ALDH2 and cytoplasmic ADH5, have greatly shortened lifespans and develop leukemia. Hematopoiesis is disrupted profoundly, with a reduction of hematopoietic stem cells and common lymphoid progenitors causing a severely depleted acquired immune system. We show that formaldehyde is a common substrate of ALDH2 and ADH5 and establish methods to quantify elevated blood formaldehyde and formaldehyde-DNA adducts in tissues. Bone-marrow-derived progenitors actively engage DNA repair but also imprint a formaldehyde-driven mutation signature similar to aging-associated human cancer mutation signatures. Furthermore, we identify analogous genetic defects in children causing a previously uncharacterized inherited bone marrow failure and pre-leukemic syndrome. Endogenous formaldehyde clearance alone is therefore critical for hematopoiesis and in limiting mutagenesis in somatic tissues. [Display omitted] •Toxic levels of genotoxic formaldehyde are produced endogenously in mammals•Two enzymes, ADH5 and ALDH2, are critical for clearance of endogenous formaldehyde•Their loss in mice and humans causes defective hematopoiesis and increased cancer•Elevated formaldehyde causes DNA damage and mutation signature found in many cancers Dingler et al. show that formaldehyde is produced endogenously at sufficient levels to induce and overwhelm DNA repair. Two enzymes, ADH5 and ALDH2, are critical in clearance of formaldehyde, whose loss results in a bone marrow failure and leukemia syndrome of purely metabolic origin.