Association of Energy and Macronutrients Intake with S-Klotho Plasma Levels in Middle-Aged Sedentary Adults: A Cross-Sectional Study

• Published in: The Journal of nutrition, health & aging health & aging, 2022, Vol.26 (4), p.360-367
• Main Authors: De-la-O, A., Jurado-Fasoli, Lucas, Gracia-Marco, L., Henriksson, P., Castillo, M. J., Amaro-Gahete, F. J.
• Format: Article
• Language: English
• Subjects: Adults; Aging; Carbohydrates; Cross-Sectional Studies; Dietary Carbohydrates; Dietary Fats; Eating; Energy; Energy Intake; Exercise; Female; Geriatrics/Gerontology; Glucuronidase; Health behavior; Humans; Klotho Proteins; Male; Medicin och hälsovetenskap; Medicine; Medicine & Public Health; Middle age; Middle Aged; Neurosciences; Nutrients; Nutrition; Original Research; Plasma; Primary Care Medicine; Quality of Life Research; Sedentary behavior
• ISSN: 1279-7707; 1760-4788; 1760-4788
• DOI: 10.1007/s12603-022-1763-2

Background The ageing process can be influenced by energy intake and different macronutrients within the diet. The soluble form of the a-Klotho gene (called S-Klotho) is widely considered as a powerful anti-ageing biomarker. Objective To analyze the association of energy, dietary energy density and macronutrient intake with S-Klotho plasma levels in middle-aged sedentary adults. Methods A total of 72 (52.8% women) middle-aged sedentary adults (53.7 ± 5.2 years old) participated in the study. Energy and macronutrients intake (i.e. fat, carbohydrate and protein) were assessed using three non-consecutive 24-h recalls. S-Klotho plasma levels were measured in the Ethylenediaminetetraacetic acid (EDTA) plasma using a solid-phase sandwich enzyme-linked immunosorbent assay. Results No association was observed between energy, dietary energy density or macronutrient intake and S-Klotho plasma levels in men (all P≥ 0.1). We found an inverse association between energy, protein and carbohydrate intake with S-Klotho plasma levels in women (all P≤0.043), which disappeared after controlling for age, lean mass index and sedentary time. An inverse association was observed between dietary energy density and S-Klotho plasma levels in women after controlling for covariates (all P≤0.05). Conclusion In summary, the present study showed an inverse association of dietary energy density with S-Klotho plasma levels in middle-aged women. In addition, our data suggest that the associations between energy and macronutrient intake could be highly dependent on lean mass and sedentary time.