Identification of Type-H-like Blood Vessels in a Dynamic and Controlled Model of Osteogenesis in Rabbit Calvarium

Angiogenesis and bone regeneration are closely interconnected processes. Whereas type-H blood vessels are abundantly found in the osteogenic zones during endochondral long bone development, their presence in flat bones’ development involving intramembranous mechanisms remains unclear. Here, we hypot...

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Bibliographic Details
Published in:Materials 2022-07, Vol.15 (13), p.4703
Main Authors: Marger, Laurine, Liaudet, Nicolas, Scherrer, Susanne S., Brembilla, Nicolo-Constantino, Preynat-Seauve, Olivier, Manoil, Daniel, Mekki, Mustapha, Durual, Stéphane
Format: Article
Language:English
Subjects:
Angiogenesis
Animals
Biomedical materials
Biopsy
Blood vessels
Bones
Capillaries
Growth factors
Hybridization
Medicin och hälsovetenskap
Regeneration (physiology)
Staining
Stem cells
Sutures
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Summary:Angiogenesis and bone regeneration are closely interconnected processes. Whereas type-H blood vessels are abundantly found in the osteogenic zones during endochondral long bone development, their presence in flat bones’ development involving intramembranous mechanisms remains unclear. Here, we hypothesized that type-H-like capillaries that highly express CD31 and Endomucin (EMCN), may be present at sites of intramembranous bone development and participate in the control of osteogenesis. A rabbit model of calvarial bone augmentation was used in which bone growth was controlled over time (2–4 weeks) using a particulate bone scaffold. The model allowed the visualization of the entire spectrum of stages throughout bone growth in the same sample, i.e., active ossification, osteogenic activity, and controlled inflammation. Using systematic mRNA hybridization, the formation of capillaries subpopulations (CD31–EMCN staining) over time was studied and correlated with the presence of osteogenic precursors (Osterix staining). Type-H-like capillaries strongly expressing CD31 and EMCN were identified and described. Their presence increased gradually from the regenerative zone up to the osteogenic zone, at 2 and 4 weeks. Type-H-like capillaries may thus represent the initial vascular support encountered in flat bones’ development and which organize osteogenic niches.
ISSN:1996-1944
1996-1944
DOI:10.3390/ma15134703