Microfluidic Device for Patient-Centric Multiplexed Assays with Readout in Centralized Laboratories

• Published in: Analytical chemistry (Washington) 2023-01, Vol.95 (2), p.1350-1358
• Main Authors: Hauser, Janosch, Dale, Matilda, Beck, Olof, Schwenk, Jochen M., Stemme, Göran, Fredolini, Claudia, Roxhed, Niclas
• Format: Article
• Language: English
• Subjects: Biohazards; Biomarkers; C-reactive protein; Chemistry; Humans; Immunoassay; Insulin-like growth factor-binding protein 1; Lab-On-A-Chip Devices; Laboratories; Medicin och hälsovetenskap; Microfluidic Analytical Techniques; Microfluidic devices; Microfluidics; Multiplexing; Patient-Centered Care; Reliability analysis; Reproducibility of Results; S100b protein; Sampling; Workflow
• ISSN: 0003-2700; 1520-6882; 1520-6882
• DOI: 10.1021/acs.analchem.2c04318

Patient-centric sampling strategies, where the patient performs self-sampling and ships the sample to a centralized laboratory for readout, are on the verge of widespread adaptation. However, the key to a successful patient-centric workflow is user-friendliness, with few noncritical user interactions, and simple, ideally biohazard-free shipment. Here, we present a capillary-driven microfluidic device designed to perform the critical biomarker capturing step of a multiplexed immunoassay at the time of sample collection. On-chip sample drying enables biohazard-free shipment and allows us to make use of advanced analytics of specialized laboratories that offer the needed analytical sensitivity, reliability, and affordability. Using C-Reactive Protein, MCP1, S100B, IGFBP1, and IL6 as model blood biomarkers, we demonstrate the multiplexing capability and applicability of the device to a patient-centric workflow. The presented quantification of a biomarker panel opens up new possibilities for e-doctor and e-health applications.