Spatial immunophenotyping of the tumour microenvironment in non–small cell lung cancer

• Published in: European journal of cancer (1990) 2023-05, Vol.185, p.40-52
• Main Authors: Backman, Max, Strell, Carina, Lindberg, Amanda, Mattsson, Johanna S.M., Elfving, Hedvig, Brunnström, Hans, O'Reilly, Aine, Bosic, Martina, Gulyas, Miklos, Isaksson, Johan, Botling, Johan, Kärre, Klas, Jirström, Karin, Lamberg, Kristina, Pontén, Fredrik, Leandersson, Karin, Mezheyeuski, Artur, Micke, Patrick
• Format: Article
• Language: English
• Subjects: Cancer and Oncology; Cancer och onkologi; Carcinoma, Non-Small-Cell Lung - pathology; CD8-Positive T-Lymphocytes; Checkpoint therapy; Clinical Medicine; Humans; Immune cell infiltration; Immunophenotyping; Klinisk medicin; Lung cancer; Lung Neoplasms - pathology; Medical and Health Sciences; Medicin och hälsovetenskap; Multiplex imaging; NSCLC; Prognosis; Tumor Microenvironment; Tumour microenvironment
• ISSN: 0959-8049; 1879-0852; 1879-0852
• DOI: 10.1016/j.ejca.2023.02.012

Immune cells in the tumour microenvironment are associated with prognosis and response to therapy. We aimed to comprehensively characterise the spatial immune phenotypes in the mutational and clinicopathological background of non–small cell lung cancer (NSCLC). We established a multiplexed fluorescence imaging pipeline to spatially quantify 13 immune cell subsets in 359 NSCLC cases: CD4 effector cells (CD4-Eff), CD4 regulatory cells (CD4-Treg), CD8 effector cells (CD8-Eff), CD8 regulatory cells (CD8-Treg), B-cells, natural killer cells, natural killer T-cells, M1 macrophages (M1), CD163+ myeloid cells (CD163), M2 macrophages (M2), immature dendritic cells (iDCs), mature dendritic cells (mDCs) and plasmacytoid dendritic cells (pDCs). CD4-Eff cells, CD8-Eff cells and M1 macrophages were the most abundant immune cells invading the tumour cell compartment and indicated a patient group with a favourable prognosis in the cluster analysis. Likewise, single densities of lymphocytic subsets (CD4-Eff, CD4-Treg, CD8-Treg, B-cells and pDCs) were independently associated with longer survival. However, when these immune cells were located close to CD8-Treg cells, the favourable impact was attenuated. In the multivariable Cox regression model, including cell densities and distances, the densities of M1 and CD163 cells and distances between cells (CD8-Treg–B-cells, CD8-Eff–cancer cells and B-cells–CD4-Treg) demonstrated positive prognostic impact, whereas short M2–M1 distances were prognostically unfavourable. We present a unique spatial profile of the in situ immune cell landscape in NSCLC as a publicly available data set. Cell densities and cell distances contribute independently to prognostic information on clinical outcomes, suggesting that spatial information is crucial for diagnostic use. •Immune cells showed specific infiltration patterns within the tumour microenvironment.•Infiltration of lymphocytes and plasmacytoid dendritic cells are associated with good prognosis.•Distances of immune cells to each other or to tumour cells reveal an independent prognostic impact.