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Shigella flexneri type-specific antigen V: cloning, sequencing and characterization of the glucosyl transferase gene of temperate bacteriophage SfV
With lysogeny by bacteriophage SfV, Shigella flexneri serotype Y is converted to serotype 5a. The glucosyl transferase gene ( gtr) from bacteriophage SfV of S. flexneri, involved in serotype-specific conversion, was cloned and characterized. The DNA sequence of a 3.7 kb EcoRI– BamHI fragment of bact...
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Published in: | Gene 1997-08, Vol.195 (2), p.207-216 |
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Main Authors: | , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | With lysogeny by bacteriophage SfV,
Shigella flexneri serotype Y is converted to serotype 5a. The glucosyl transferase gene (
gtr) from bacteriophage SfV of
S. flexneri, involved in serotype-specific conversion, was cloned and characterized. The DNA sequence of a 3.7
kb
EcoRI–
BamHI fragment of bacteriophage SfV which includes the
gtr gene was determined. This gene, encoding a polypeptide of 417
aa with 47.67
kDa molecular mass, caused partial serotype conversion of
S. flexneri from serotype Y to type V antigen as demonstrated by Western blotting and the sensitivity of the hybrid strain to phage Sf6. The deduced protein of the partially sequenced open reading frame upstream of the
gtr showed similarity to various glycosyl transferases of other bacteria.
Orf3, separated from the
gtr by a non-coding region and transcribed convergently, codes for a 167
aa (18.8
kDa) protein found to have homology with tail fibre genes of phage lambda and P2. |
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ISSN: | 0378-1119 1879-0038 |
DOI: | 10.1016/S0378-1119(97)00144-3 |